Ketoprofen Associated with Hyaluronic Acid Hydrogel for Temporomandibular Disorder Treatment: An In Vitro Study.

Temporomandibular disorders (TMD) are a public health problem that affects around 12% of the global population. The treatment is based on analgesics, non-steroidal anti-inflammatory, corticosteroids, anticonvulsants, or arthrocentesis associated with hyaluronic acid-based viscosupplementation. However, the use of hyaluronic acid alone in viscosupplementation does not seem to be enough to regulate the intra-articular inflammatory process.

Analysis of the effect of photodynamic therapy with Fotoenticine on gliosarcoma cells.

Gliosarcoma is a highly aggressive malignant neoplasm and a histopathological variant of wild-type glioblastoma multiforme isocitrate dehydrogenase (HDI). The current standard treatment consists of chemotherapy, radiotherapy and surgical resection, however, despite advances in these techniques, the patient's prognosis remains unfavorable. Photodynamic therapy (PDT) is a noninvasive technique that has been highlighted as an alternative form of cancer treatment because it does not present the side effects associated with systemic treatments.

The effect of low-level laser therapy (660 nm) on the gene expression involved in tissue repair.

The effect of low-level laser therapy (LLLT) on the healing of skin lesions has been evaluated in many studies; however, the molecular mechanisms involved in the biostimulatory effects resulting from this treatment need to be better understood. The paper aims to analyze the effects of LLLT (660 nm) at doses of 1 and 5 J/cm on cell viability and expression of vascular endothelial growth factor (VEGF) and interleukin (IL6) genes in L929 fibroblast cells. The dose-response curve was performed with the GaInAlAs (660 nm) laser-treated cells at energy rates of 1 and 5 J/cm.

Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity.

Objectives: This study evaluated the biological effects of the T. vulgaris L. extract.

Photodithazine photodynamic effect on viability of 9L/lacZ gliosarcoma cell line.

Even with the advances of conventional treatment techniques, the nervous system cancer prognosis is still not favorable to the patient which makes alternative therapies needed to be studied. Photodynamic therapy (PDT) is presented as a promising therapy, which employs a photosensitive (PS) agent, light wavelength suitable for the PS agent, and molecular oxygen, producing reactive oxygen species in order to induce cell death. The aim of this study is to observe the PDT action in gliosarcoma cell using a chlorin (Photodithazine, PDZ).

Plant extracts: initial screening, identification of bioactive compounds and effect against Candida albicans biofilms.

Aim: This study screened plants from Brazilian Pantanal for Candida albicans antibiofilm activity.

Material & Methods: Sixty extracts were obtained from ten plants using different extraction methods. Antifungal activity was assessed.

Effect of photodynamic therapy supplemented with quercetin in HEp-2 cells.

Photodynamic therapy (PDT) is a technique that can be used as a complementary therapy in cancer treatment combined with other therapeutic modalities. Quercetin (QCT) is known to be effective in the treatment of cancer, by reducing the cell viability of different cancer cell lines. This study aimed to evaluate the influence of different concentrations of QCT in PDT on the viability, mitochondrial membrane potential and induction of apoptosis/necrosis in the human larynx carcinoma cells (HEp-2).

Effect of low-intensity pulsed ultrasound on l929 fibroblasts.

Introduction: Ultrasound has proven to be an important therapeutic resource regarding musculoskeletal disease and is routinely used in physical therapy and medicine both therapeutically and diagnostically. The aim of the present study was to analyse the effects with different ultrasound intensities in order to establish the ideal radiation level in cell cultures.

Material And Methods: FIBROBLAST CELL CULTURES WERE DIVIDED INTO FIVE GROUPS: group I - control (did not receive irradiation); group II - 0.

Assessment of the genetic risks of a metallic alloy used in medical implants.

The use of artificial implants provides a palliative or permanent solution for individuals who have lost some bodily function through disease, an accident or natural wear. This functional loss can be compensated for by the use of medical devices produced from special biomaterials. Titanium alloy (Ti-6Al-4V) is a well-established primary metallic biomaterial for orthopedic implants, but the toxicity of the chemical components of this alloy has become an issue of concern.

Assessment of cytoskeleton and endoplasmic reticulum of fibroblast cells subjected to low-level laser therapy and low-intensity pulsed ultrasound.

Objective: The aim of the present study was to compare the effect of low-level laser therapy (LLLT) and low-intensity pulsed ultrasound (LIPUS) on the cytoskeleton and endoplasmic reticulum of L929 cells. Thermal and non-thermal physical mechanisms such as LLLT and LIPUS induce clinically significant responses in cells, tissues, and organs.

Materials And Methods: L929 fibroblast cell cultures were irradiated with LLLT and subjected to LIPUS.

Effects of a polarized light source (400-2000 nm) on Hep.2 and L929 cell lines: a spectroscopic in vitro study.

Objective: The aim of this study was to evaluate the effects on Hep.2 cells originating from laryngeal carcinomas, and L929 cells originating from a fibroblast line, subjected to polarized light at a wavelength of 400-2000 nm.

Background Data: Recently there has been increased interest in the propagation of polarized light in randomly scattering media such as biological tissues, because of its potential applications in medicine.

Cytotoxicity of octal-bromide zinc phthalocyanine after photodynamic therapy with different light sources.

Objective: The objective of this study was to investigate the cytotoxicity of octal-bromide zinc phthalocyanine (ZnPcBr8) at different concentrations (0.25, 0.5, and 1 microM) after irradiating HEp-2 cell cultures with two different light sources: a diode semiconductor laser (660 nm, 30 mW) or an LED (640 nm, 70 mW).

Evaluation of low-level laser therapy of osteoblastic cells.

Objective: The purpose of the present study was to evaluate the effect of biomodulation on osteoblastic cells using a gallium-aluminium-arsenide diode laser.

Background Data: Low-level laser therapy (LLLT) is a non-pharmacological therapeutic resource to which biological tissues respond well, producing such effects as the acceleration of bone formation and bone repair.

Materials And Methods: Osteoblastic cell cultures (OFCOL II) were irradiated with a gallium-aluminium-arsenide diode laser (GaAlAs lambda = 830 nm; 50 mW; 3 J/cm(2); 600-microm-diameter optical fiber) and divided into two groups: group 1--irradiated cells, and group 2--non-irradiated cells.

Comparison between the effect of low-level laser therapy and low-intensity pulsed ultrasonic irradiation in vitro.

Objective: The objective of this study was to compare the effect of low-level laser therapy (LLLT) and low-intensity pulsed ultrasound (LIPUS) on fibroblast cell culture. Several methods, including ultrasound treatment and LLLT, are being used to facilitate tissue repair and healing processes.

Materials And Methods: L929 fibroblast cell cultures were irradiated with low-level laser energy and LIPUS.

Surface morphology of sound deciduous tooth enamel after application of a photo-absorbing cream and infrared low-level laser irradiation: an in vitro scanning electron microscopy study.

Objectives: The purpose of this descriptive scanning electron microscopic study was to characterize surface alterations in deciduous tooth enamel after in vitro infrared diode laser irradiation, using a photo-absorbing agent alone and also combined with fluoride, before and after laser irradiation.

Background Data: Previous investigations have demonstrated increased enamel caries resistance after laser irradiation.

Methods: Seven extracted or exfoliated primary molar teeth underwent soft tissue débridement and fluoride-free prophylaxis.

Photodynamic therapy with a new photosensitizing agent.

Objective: The aim of this study was to investigate the cytotoxicity of octal-bromide zinc phthalocyanine (ZnPcBr(8)) before and after irradiation with a low-power laser (AsGaAl) and analyze the effects of photodynamic therapy (PDT) on the nucleus of L929 cells.

Background Data: One of the most recent and promising applications of phthalocyanine in medicine is in the detection and cure of tumors. We studied the ZnPcBr(8) in agreement with the development of new photosensitizing agents for curing tumors.

The effect of laser therapy on the proliferation of oral KB carcinoma cells: an in vitro study.

Objective: The aim of this study was to assess the proliferative effect of carcinoma cells, strain KB, submitted to laser therapy with wavelengths of lambda685nm (31 mW; Ø; 0.38 cm(2), 4 J/cm(2)) or lambda830nm (34.5 mW; Ø; 0.

Laser light is capable of inducing proliferation of carcinoma cells in culture: a spectroscopic in vitro study.

Objective: The objective of this work was to evaluate the influence of time, treatment, and wavelength, through the assessment of the cellular viability with MTT, on the proliferation of H.Ep.2 cells subjected to laser irradiation or not (lambda685 and lambda830 nm) with the same energy density (4 J/cm2).

Laser light prevents apoptosis in Cho K-1 cell line.

Objective: The present study investigated the effects of low-level laser therapy (LLLT) on the mitochondria, nucleus, and cytoskeleton of CHO K-1 cells by the use of specific fluorescent probes.

Background Data: The use of LLLT has been recommended by several authors for acceleration of the healing process. The literature on the effects of LLLT in this process is highly contradictory because of difficulties in identifying its effects on cells.