A Photo-Caged Cross-Linker for Identifying Protein-Protein Interactions.

Cross-linking mass spectrometry (XL-MS) has seen significant improvements which have enhanced its utility for studying protein-protein interactions (PPIs), primarily due to the emergence of novel crosslinkers and the development of streamlined analysis workflows. Nevertheless, poor membrane permeability and side reactions with water limit the extent of productive intracellular crosslinking events that can be achieved with current crosslinkers. To address these problems, we have synthesized a novel crosslinker with o-nitrobenzyl-based photoresponsive groups.

Caged lipid probes for controlling lipid levels on subcellular scales.

Lipids exert their cellular functions in individual organelles, in some cases on the scale of even smaller, specialized membrane domains. Thus, the experimental capacity to precisely manipulate lipid levels at the subcellular level is crucial for studying lipid-related processes in cell biology. Photo-caged lipid probes which partition into specific cellular membranes prior to photoactivation have emerged as key tools for localized and selective perturbation of lipid concentration in living cells.

International Study of Asthma and Allergy in Childhood Phase III (ISAAC III): The Role of Non-Response in Valencia.

A study was done about non-participation during Phase III of the International Study of Asthma and Allergy in Childhood (ISAAC III) in Valencia to determine whether the non-response rate significantly affected the results obtained in Valencia. Of the schools selected to participate in ISAAC III, 13.3% denied not to, the main reason being they were already participated in a similar study.