Meniscal Replacement With a Silk Fibroin Scaffold Reduces Contact Stresses in the Human Knee.

The aim of the current study was to verify if a previously developed silk fibroin scaffold for meniscal replacement is able to restore the physiological distribution of contact pressure (CP) over the articulating surfaces in the human knee joint, thereby reducing peak loads occurring after partial meniscectomy. The pressure distribution on the medial tibial articular surface of seven human cadaveric knee joints was analysed under continuous flexion-extension movements and under physiological loads up to 2,500 N at different flexion angles. Contact area (CA), maximum tibiofemoral CP, maximum pressure under the meniscus and the pressure distribution were analysed for the intact meniscus, after partial meniscectomy as well as after partial medial meniscal replacement using the silk fibroin scaffold.

Articular cartilage and meniscus reveal higher friction in swing phase than in stance phase under dynamic gait conditions.

Most previous studies investigated the remarkably low and complex friction properties of meniscus and cartilage under constant loading and motion conditions. However, both load and relative velocity within the knee joint vary considerably during physiological activities. Hence, the question arises how friction of both tissues is affected by physiological testing conditions occurring during gait.

The challenge of implant integration in partial meniscal replacement: an experimental study on a silk fibroin scaffold in sheep.

Purpose: To restore meniscal function after excessive tissue damage, a silk fibroin implant for partial meniscal replacement was developed and investigated in an earlier sheep model. After 6 months implantation, it showed promising results in terms of chondroprotection and biocompatibility. To improve surgical fixation, the material was subjected to optimisation and a fibre mesh was integrated into the porous matrix.

Peptide-directed spatial organization of biomolecules in dynamic gradient scaffolds.

Specific binding peptides are used to spatially organize biomolecule gradients within an electrospun fiber scaffold. Different biomolecule-binding peptide-polymer conjugates are sequentially co-electrospun with a fiber-forming host polymer to generate opposing gradients of peptide functionalization. The binding peptides specifically and non-covalently guide the spatial arrangement of biomolecules into dynamic gradients within the scaffold, mimicking biological gradients found in native tissues.

Responsive poly (γ-glutamic acid) fibres for biomedical applications.

A novel responsive system using a protein-based biopolymer was designed to undergo structural, geometric, and chemical changes upon temperature change or solvent interaction. Poly(γ-glutamic acid) (γ-PGA) is an attractive candidate for various biomedical applications as it is naturally produced, biocompatible and enzymatically degradable. The responsive material was fabricated using an electrospun modified γ-PGA to create a sub-micron fibrous mat.

Functionalized poly(γ-Glutamic Acid) fibrous scaffolds for tissue engineering.

Poly(γ-glutamic acid) (γ-PGA) is a biocompatible, enzymatically-degradable, natural polymer with a higher resistance to hydrolysis than polyesters commonly used for tissue engineering scaffolds such as poly(L-lactide) (PLLA). Notably, γ-PGA's free carboxyl side groups allow for simple chemical functionalization, making it a versatile candidate for producing scaffolds. Here, a series of water-resistant fibrous scaffolds were engineered from ethyl (Et), propyl (Pr) and benzyl (Bn) esterifications of γ-PGA.

Self-organization of mixtures of fluorocarbon and hydrocarbon amphiphilic thiolates on the surface of gold nanoparticles.

Self-assembled monolayers composed of a mixture of thiolate molecules, featuring hydrocarbon or perfluorocarbon chains (H- and F-chains) terminating with a short poly(oxoethylene) (PEG) moiety, are the most extreme example of surfactant immiscibility on gold nanoparticles reported so far. The phase segregation between H-chains and F-chains and the consequent, peculiar folding of PEG chains are responsible for the increased affinity of a selected radical probe for the fluorinated region, which increases as the size of the fluorinated domains decrease, independently of the shape of such domains. This feature has been revealed by ESR measurements and an in silico innovative multiscale molecular simulations approach in explicit water.

Formation of patches on 3D SAMs driven by thiols with immiscible chains observed by ESR spectroscopy.

Beyond stripes: The extreme lipophobicity of perfluorinated chains attached to amphiphilic thiolates triggers the formation of "stars" (or patches) surrounded by amphiphilic alkylthiolates in three-dimensional self-assembled monolayers. This strategy led to the first example of a water-soluble multicompartment monolayer wrapped around a gold core.

Water-soluble gold nanoparticles protected by fluorinated amphiphilic thiolates.

The preparation and the properties of gold nanoparticles (Au NPs) protected by perfluorinated amphiphiles are described. The thiols were devised to form a perfluorinated region close to the gold surface and to have a hydrophilic portion in contact with the bulk solvent to impart solubility in water. The monolayer protected clusters were prepared, in an homogeneous phase using sodium thiolates because of the low nucleophilicity of the alpha-perfluorinated thiols, and fully characterized with (1)H, (19)F NMR spectrometry, IR and UV-vis absorption spectroscopies, transmission electron microscopy (TEM), thermogravimetric analysis (TGA), and X-ray photoelectron spectroscopy (XPS).

Effect of core size on the partition of organic solutes in the monolayer of water-soluble nanoparticles: an ESR investigation.

ESR spectroscopy has been used to study the interaction of para-pentylbenzyl hydroxyalkyl nitroxide with the monolayer of water-soluble protected gold clusters having a core diameter ranging from 1.6 to 5.3 nm.