West Nile Virus and Related Flavivirus in European Wild Boar (), Latium Region, Italy: A Retrospective Study.

Background: A retrospective sero-survey for evidence of West Nile virus (WNV) infection in European wild boar () was conducted in the Latium region, Italy, on stored serum samples of the period November 2011 to January 2012.

Methods: Sera were collected from 168 European wild boars and screened for antibodies to WNV and other Flaviviruses by competitive enzyme linked immunosorbent assay (cELISA). All sera positive for Flavivirus antibodies by cELISA were further examined by virus neutralization test (VNT).