Cytometric bead assay of cytokines in sepsis: a clinical evaluation.

Background: The clinical relevancy of an attractive new multiparametric method, cytometric beads assay (CBA), was evaluated for the monitoring of cytokines in sepsis.

Methods: A total of 52 samples (26 patients) were simultaneously tested by CBA and chemiluminescence (IL-6, IL-8, IL-1beta, and TNFalpha) or ELISA (IL-10, IL-12 p40, IL-12 p70, and soluble TNFalpha-RI).

Results: CBA standard curves were linear from 20-5,000 ng/L except for IL-1beta (40-5,000 ng/L).