Publications by authors named "Cristiane G M Pinheiro"

rIL-10 plays a major role in restricting exaggerated inflammatory and immune responses, thus preventing tissue damage. However, the restriction of inflammatory and immune responses by IL-10 can also favor the development and/or persistence of chronic infections or neoplasms. Dogs that succumb to canine leishmaniasis (CanL) caused by L.

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Leishmania infantum causes from subclinical infection to severe disease in humans and dogs. The spleen is one of the organs most affected by the infection. Although evidence exists that the parasitic load distribution and histological alterations may not be homogeneous in the affected organs of naturally infected individuals, it has not been formally demonstrated using the current techniques used for studying the disease.

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Background: Interleukin-12 is an important cytokine in mediating cellular immune responses.

Results: Recombinant single-chain canine IL-12 was produced in a baculovirus-insect cell system with the aim of conducting further studies on modulation of immune responses in dogs. To optimize the production of recombinant canine IL-12, a classical baculovirus and a modified vector (chitinase A and v-cathepsin knockout) were used containing a native or an optimized insert of canine IL-12.

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Article Synopsis
  • Very few studies focus on altering immune responses in dogs, so this research examined the effects of recombinant canine IL-2 and IL-12 fusion protein on dog blood cells.
  • Recombinant IL-2 was found to effectively stimulate the growth of canine blood cells, while the combination of IL-12 and IL-2 boosted the production of interferon gamma, an important immune signaling molecule.
  • The findings indicate that using both IL-2 and IL-12 together could enhance immune responses in dogs, which may have implications for veterinary treatments.
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To expand the available panel of recombinant proteins that can be useful for identifying Leishmania-infected dogs and for diagnosing human visceral leishmaniasis (VL), we selected recombinant antigens from L. infantum, cDNA, and genomic libraries by using pools of serum samples from infected dogs and humans. The selected DNA fragments encoded homologs of a cytoplasmic heat-shock protein 70, a kinesin, a polyubiquitin, and two novel hypothetical proteins.

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