Publications by authors named "Craig Livie"
A liquid chromatography-tandem mass spectrometry method for the analysis of dehydroepiandrosterone sulphate (DHEAs) in serum and plasma with comparison to an immunoassay method in a neonatal population.
J Chromatogr B Analyt Technol Biomed Life Sci
April 2023
The measurement of dehydroepiandrosterone-sulphate (DHEAs) is an important second-line test to aid in the diagnosis of premature adrenarche, peripubertal gynaecomastia in males and in identifying the source of elevated androgens in females. Historically, DHEAs has been measured by immunoassay platforms which are prone to poor sensitivity and more importantly poor specificity. The aim was to develop an LC-MSMS method for the measurement of DHEAs in human plasma and serum, develop an in-house paediatric (<6 year old) reference limit and compare the performance against the Abbott Alinity DHEAs immunoassay method.
View Article and Find Full Text PDFBackground: Urinary 5-hydroxyindoleacetic acid (5-HIAA) is a first-line investigation for gastrointestinal neuroendocrine tumours that secrete serotonin. It also has clinical utility for monitoring disease progression and therapeutic response.
Aim: To develop and validate a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for urinary 5-hydroxyindoleacetic acid that incorporates a supported liquid extraction and C-labelled internal standard.
The loss of normal regulation of corticosteroid secretion is important in the development of cardiovascular disease. We previously showed that microRNAs regulate the terminal stages of corticosteroid biosynthesis. Here, we assess microRNA regulation across the whole corticosteroid pathway.
View Article and Find Full Text PDFArticle Synopsis
- The cAMP/PKA signaling pathway is carefully controlled in terms of where and when signals are sent, which helps ensure specific cellular responses.
- Phosphodiesterases (PDEs), especially PDE2A2, are crucial for managing local cAMP levels at particular sites within cells, including mitochondrial membranes.
- Research shows that inhibiting PDE2A2 can boost local cAMP levels, impacting mitochondrial function and potentially protecting cells from programmed death (apoptosis).
Article Synopsis
- - The study investigates how different levels of 3'-5'-cyclic adenosine monophosphate (cAMP) influence cardiac conditions, particularly focusing on the role of specific phosphodiesterases (PDEs) in regulating cAMP levels and their effects on heart muscle cell growth.
- - Researchers found that inhibiting PDE3 and PDE4 raises cAMP and promotes heart cell hypertrophy, while inhibiting PDE2 decreases hypertrophy by creating a localized pool of cAMP that activates a specific protein kinase A subset.
- - The findings highlight PDE2 as a crucial regulator of cardiac hypertrophy, suggesting that targeting this enzyme could lead to potential therapies for heart diseases.