Apyrase (nucleoside triphosphate-diphosphohydrolase) and extracellular nucleotides regulate cotton fiber elongation in cultured ovules.

Ectoapyrase enzymes remove the terminal phosphate from extracellular nucleoside tri- and diphosphates. In Arabidopsis (Arabidopsis thaliana), two ectoapyrases, AtAPY1 and AtAPY2, have been implicated as key modulators of growth. In fibers of cotton (Gossypium hirsutum), transcript levels for GhAPY1 and GhAPY2, two closely related ectoapyrases that have high sequence similarity to AtAPY1 and AtAPY2, are up-regulated when fibers enter their rapid growth phase.

Ectopic expression of an annexin from Brassica juncea confers tolerance to abiotic and biotic stress treatments in transgenic tobacco.

Plant annexins belong to a multigene family and are suggested to play a role in stress responses. A full-length cDNA for a gene encoding an annexin protein was isolated and characterized from Brassica juncea (AnnBj1). AnnBj1 message levels were regulated by abscisic acid, ethephon, salicylic acid, and methyl jasmonate as well as chemicals that induce osmotic stress (NaCl, Mannitol or PEG), heavy metal stress (CdCl(2)) and oxidative stress (methyl viologen or H(2)O(2)).