Extremely fast construction and querying of compacted and colored de Bruijn graphs with GGCAT.

Compacted de Bruijn graphs are one of the most fundamental data structures in computational genomics. Colored compacted de Bruijn graphs are a variant built on a of sequences and associate to each -mer the sequences in which it appears. We present GGCAT, a tool for constructing both types of graphs, based on a new approach merging the -mer counting step with the unitig construction step, as well as on numerous practical optimizations.

Morphological and molecular characterization of Austrodiplostomum compactum metacercariae in the eyes and brains of fishes from the Ivaí River, Brazil.

Austrodiplostomum spp. (Platyhelminthes: Digenea) are endoparasites with a broad geographic distribution in South America. During the larval stage, they parasitize the eyes, brains, muscles, gill, kidneys and swim bladder of a wide variety of fishes.

Trends in US Kidney Transplantation During the COVID-19 Pandemic.

Solid organ transplants have been impacted significantly during the COVID-19 pandemic in the United States. Limited data exist regarding changes in living donor kidney transplants. The aim of this study was to describe national trends in kidney transplantation during COVID-19.

Cytoreductive surgery and hyperthermic intraperitoneal chemotherapy combined with two-stage hepatectomy for multiple and bilobar desmoplastic small round cell tumor liver metastases.

Desmoplastic small round cell tumor (DSRCT) is a rare mesenchymal tumor usually affecting young patients. Local dissemination is common, and liver is the most common site for extraperitoneal metastases. Multimodal management has been shown to be the most effective treatment.

Faecal incontinence in patients with a sphincter defect: comparison of sphincteroplasty and sacral nerve stimulation.

Aim: Sphincteroplasty (SP) is used to treat faecal incontinence (FI) in patients with a sphincter defect. Although sacral nerve stimulation (SNS) is used in patients, its outcome in patients with a sphincter defect has not been definitively evaluated. We compared the results of SP and SNS for FI associated with a sphincter defect.

Outcomes of Reimplantation of the Artificial Bowel Sphincter.

Background: Outcomes of artificial bowel sphincter reimplantation for severe fecal incontinence remain unknown.

Objective: The purpose of this study was to evaluate the feasibility and outcomes of artificial bowel sphincter reimplantation versus implantation.

Design: This was a retrospective review study.

Outcomes of Sacral Neurostimulation Lead Reimplantation for Fecal Incontinence: A Cohort Study.

Background: Adverse events and complications have been reported after sacral neurostimulation for fecal incontinence, which may result in surgical revision and device explantation. Lead reimplantation may be feasible; however, available data regarding outcomes are less robust.

Objective: The aim of this study was to determine the outcomes of sacral neurostimulation lead reimplantation for fecal incontinence.

Basketball injuries: caring for a basketball team.

Basketball is a popular sport in North America and worldwide. Most injuries are lower extremity injuries to the ankle and knee. In this article, injuries common to basketball and, from our experience, injuries that escape injury surveillance systems are discussed from the physician and athletic trainer's perspective.

Determinants of on-the-job-barriers in employed persons with multiple sclerosis: the role of disability severity and cognitive indices.

Background: Literature has shown that work maintenance is central in order to guarantee participation to persons with disability. Knowledge about potential sources of difficulties and obstacles is then crucial in order to prevent barriers and facilitate work maintenance and career development for persons with disabilities.

Objective: Studies analyzing on-the-job barriers among employed people with multiple sclerosis (MS) have found evidence for a role of clinical determinants.

Chordal cutting technique through aortotomy to treat chronic ischemic mitral regurgitation: surgical technique.

Objective: To describe original surgical treatment in patients with ischemic mitral valve regurgitation due to tenting phenomenon.

Background: The optimal surgical treatment of ischemic mitral regurgitation (MR) in patients with coronary artery disease is controversial. The standard treatment is revascularization and reduction annuloplasty.

Retrospective study of children with acute pyelonephritis. Evaluation of bacterial etiology, antimicrobial susceptibility, drug management and imaging studies.

The purpose of the study was to investigate the management of pyelonephritis in a large Italian pediatric population. A total of 1,333 patients (36% male) were considered. Escherichia coli was the most frequently isolated agent (89.

The Pediatric Bowel Management Clinic in children with severe chronic constipation. Personal experience.

The multifactorial nature of functional constipation in children suggests that a multidisciplinary management approach may be effective and leads to both patients and parents' satisfaction and significant short term improvement. The authors describe their experience with the study and treatment of children with severe chronic constipation in a newly-created Pediatric Bowel Management Clinic (BMC). Further studies will examine the long-term impact of the clinic.

Interstitial lung disease in children: a multicentre survey on diagnostic approach.

Chronic interstitial lung disease (ILD) is a rare disorder in the paediatric age group, with a poor prognosis. The diagnostic approach to ILD is based on more or less invasive methods. This study was implemented to verify which methods are the most often used in children.

Reference values of exhaled nitric oxide for healthy children 6-15 years old.

Nitric oxide (NO) can be detected in human exhaled air, and its endogenous production is increased in patients with asthma. It may provide a noninvasive means for measuring airway inflammation. The aim of this study was to establish reference values for exhaled NO concentrations in a large number of healthy school-age children.

Use of the paediatric bronchoscope, flexible and rigid, in 51 European centres.

We have undertaken a survey to establish current practices and differences in the use of bronchoscopes in children in European centres. A questionnaire was sent to all 220 members of the Paediatric Assembly of the European Respiratory Society (ERS). The questions concerned the following points: indications for bronchoscopy; site of bronchoscopy; type of sedation; any oxygen supplementation during the procedure; number of procedures performed in the previous 12 months; number of procedures performed in the neonatal intensive care unit; number of bronchoalveolar lavages (BALs); side-effects during and after the procedures; and diagnostic yield.

Determination of inulin in plasma and urine by reversed-phase high-performance liquid chromatography.

We report a new HPLC procedure for measuring inulin in plasma and urine. Samples after dilution are boiled in mild acidic conditions and then analyzed on a C18 column. Solvent system A is 3.

The first 20 minutes after a single dose of inhaled salmeterol in asthmatic children.

Very little is known as yet about the effect of salmeterol in pediatric asthma, so a trial was performed on children with mild asthma to compare salmeterol with salbutamol in terms of how quickly they took effect. The double-blind study involved 11 children (mean age 13.4 years) randomly assigned to inhale salmeterol 50 micrograms, salbutamol 200 micrograms, or a placebo three times on alternate days.

Determination of 8-methoxypsoralen in plasma by gas chromatography-mass spectrometry using selected-ion monitoring.

A sensitive and accurate assay was developed for the measurement of 8-methoxypsoralen in plasma using electron-impact positive-ion mass fragmentography. 4,5,8-Trimethylpsoralen was used as an internal standard. Sample preparation consisted of a two-step liquid phase extraction using acetonitrile and methylene chloride.

Determination of urinary orotate excretion by high-performance liquid chromatography.

A new reversed-phase high-performance liquid chromatographic procedure for the determination of urinary orotate excretion is described. It is a selective, sensitive and rapid method, suitable for the differentiation of inherited metabolic diseases with abnormal orotate metabolism.

[Psychological study of adults surgically treated in childhood for hypospadias].

The authors present the study and results in 100 subjects operated of hypospadia. They interviewed patients within 15 and 22 years old, in randomized selection and in permanent percent relations as far as: different types of pathology, the age of surgery effected in Vicenza Hospital - Pediatric Surgery Department (1971-1981). Through a structured questionnaire they tried to analyse psychological lived in operated subjects as far as: surgery, convalescence, psychologic problems before and after surgery, way of life when they became adults.

[Cardiosurgical intervention in extracorporeal circulation and intellectual performance in childhood. Study and follow-up of 20 cases].

6 and 12 months before and after surgery in E.C.C.

[Sound alarms and conditioning therapy in the treatment of childhood enuresis: a study of 180 cases].

The Authors present the study and results in 180 cases of infantile enuresis, treated with a new bell alarm associated to conditioning psychological treatment (toilet training, token economy, motivational counseling, urine stop exercises, etc.). The cases have been subdivided into the type of enuresis, age and sex.

[Biofeedback in asthmatic children].

This paper reports the results of the treatment of continuous bronchial asthma in children, 7-14 years old, by means of biofeedback and the counter-conditioning. We observed the remission of the symptomatology in all the cases, with a statistical significance.

[The spina bifida patient and his family: psychologic and social aspects. 2].

In the second part of the present work the data which was obtained through questionnaires and interviews with 130 families with children affected by spina bifida is presented. Presented first are the immediate sociopsychological aspects of the family when they are informed about the malformation, the derived psychological effects and the preoccupations relating to the life of their child. Then the aspects of the successive social and psychological problems of these parents are presented, and finally the expectations and demands of the same parents following the psychological operations carried out at the centre to the benefit of the children and their families.

[The subject with spina bifida and his family: psychological and social aspects. I].

This paper is to examine the psychological and social effects in spina bifida. In 130 subjects, aged from 4 to 24 years old, the levels of the I.Q.