E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for AAV production in HEK293 cells.

The replication-defective adeno-associated virus (AAV) is extensively utilized as a research tool or vector for gene therapy. The production process of AAV remains intricate, expensive, and mechanistically underexplored. With the aim of enhancing AAV manufacturing efficiencies in mammalian cells, we revisited the questions and optimization surrounding the requirement of the various adenoviral helper genes in enabling AAV production.

Two Decades of Gastrointestinal Stromal Tumor Management With First-Line Treatment: A Case Report.

Gastrointestinal stromal tumors (GISTs) are rare malignant tumors that arise from the connective tissues of the gastrointestinal tract. Reports about GISTs treated with imatinib for over five years are exceedingly rare. In this case report, we present a patient with GIST who remained alive for two decades after undergoing imatinib treatment.

The end of an entire biome? World's largest wetland, the Pantanal, is menaced by the Hidrovia project which is uncertain to sustainably support large-scale navigation.

The resurgent navigation project known as the Hidrovia Paraguay-Paraná threatens the integrity of the Pantanal, the world's largest wetland, which is considered a biome of its own. Intensive barge navigation is intended to transport crops (soybean, sugar, corn) and cement, iron and manganese from areas of production in Brazil, Paraguay and Bolivia to the oceanic ports of the Plata River. This short communication assembles the information available on the potential impacts of the planned deepening of the natural channel of the Paraguay River in its ~700 km-long upper section.

Detection of Mycobacterium bovis in Free-Ranging Sapajus nigritus, Argentina.

Mycobacterium bovis and Mycobacterium tuberculosis are the most relevant among pathogenic mycobacteria, both belonging to the M. tuberculosis complex (MTC). Samples of blood, liver, spleen, kidneys, lungs and caseous tubercles were collected from a free-ranging juvenile black capuchin monkey (Sapajus nigritus) showing non-specific signs of illness.

Municipal and industrial wastewater blending: Effect of the carbon/nitrogen ratio on microalgae productivity and biocompound accumulation.

Municipal wastewater (MW) and industrial wastewater from juice processing (IWJ) were blended in different proportions to assess the effect of the carbon/nitrogen (C/N) ratio on pollutant removal, microalgal biomass (MB) cultivation, and the accumulation of carotenoids and biocompounds. MB development was not observed in treatments with higher C/N ratios (>30.67).

Training set optimization is a feasible alternative for perennial orphan crop domestication and germplasm management: an example.

Orphan perennial native species are gaining importance as sustainability in agriculture becomes crucial to mitigate climate change. Nevertheless, issues related to the undomesticated status and lack of improved germplasm impede the evolution of formal agricultural initiatives. - neotropical palm with potential for oil production - is an example.

Boost and Increased Antibody Breadth Following a Second Dose of PARVAX for SARS-CoV-2 in Mice and Nonhuman Primates.

PARVAX is a genetic vaccine platform based on an adeno-associated vector that has demonstrated to elicit potent, durable, and protective immunity in nonhuman primates (NHPs) after a single dose. Here, we assessed vaccine immunogenicity following a PARVAX prime-boost regimen against SARS-CoV-2. In mice, a low-dose prime followed by a higher-dose boost elicited potent neutralizing antibody responses and distinct cross-reactivity profiles, depending on the antigen used in the booster vaccine.

Biofuel from wastewater-grown microalgae: A biorefinery approach using hydrothermal liquefaction and catalyst upgrading.

Third-generation biofuels from microalgae are becoming necessary for sustainable energy. In this context, this study explores the hydrothermal liquefaction (HTL) of microalgae biomass grown in wastewater, consisting of 30% Chlorella vulgaris, 69% Tetradesmus obliquus, and 1% cyanobacteria Limnothrix planctonica, and the subsequent upgrading of the produced bio-oil. The novelty of the work lies in integrating microalgae cultivation in wastewater with HTL in a biorefinery approach, enhanced using a catalyst to upgrade the bio-oil.

["God for us!": Catholic festivals and rites in times of epidemics, Salvador-BA, Brasil, 1918-1919].

This article analyzes the reactions of Catholics linked to lay associations in the city of Salvador, in the period of the Spanish flu (1918) and smallpox (1919). Newspapers were the main sources used to identify the festivals and rites, both those practiced to ask for the intercession of the saints, and those that were suspended due to the need for social isolation. In spite of both diseases being transmissible and the short interval between the two epidemics, the analysis of the sources showed different reactions from the faithful regarding the measures of protection and the search for a cure.

Recommendation of Tahiti acid lime cultivars through Bayesian probability models.

Probabilistic models enhance breeding, especially for the Tahiti acid lime, a fruit essential to fresh markets and industry. These models identify superior and persistent individuals using probability theory, providing a measure of uncertainty that can aid the recommendation. The objective of our study was to evaluate the use of a Bayesian probabilistic model for the recommendation of superior and persistent genotypes of Tahiti acid lime evaluated in 12 harvests.

Biocompounds from wastewater-grown microalgae: a review of emerging cultivation and harvesting technologies.

Microalgae biomass has attracted attention as a feedstock to produce biofuels, biofertilizers, and pigments. However, the high production cost associated with cultivation and separation stages is a challenge for the microalgae biotechnology application on a large scale. A promising approach to overcome the technical-economic limitations of microalgae production is using wastewater as a nutrient and water source for cultivation.

The perfect storm: Cutaneous leishmaniasis in troops deployed in the Atlantic forest of Argentina.

American cutaneous leishmaniasis (ACL) is the most prevalent form of leishmaniasis, associated with an ulcerative and stigmatizing mucocutaneous pathology. This study assessed the incidence of Leishmania (Viannia) braziliensis in members of the Argentine Army who were exposed to sandfly bites in Iguazú National Park (INP), northeastern Argentina, during an outbreak of ACL in 2019, and the presence of Leishmania in rodents, opossums and phlebotomine sandflies collected in the area of exposure. Samples from military personnel, wild animals and phlebotomine sandflies were analysed.

Portuguese Consensus on Acute Porphyrias: Diagnosis, Treatment, Monitoring and Patient Referral.

Acute porphyrias are a group of rare genetic metabolic disorders, caused by a defect in one of the enzymes involved in the heme biosynthesis, which results in an abnormally high accumulation of toxic intermediates. Acute porphyrias are characterized by potentially life-threatening attacks and, for some patients, by chronic manifestations that negatively impact daily functioning and quality of life. Clinical manifestations include a nonspecific set of gastrointestinal, neuropsychiatric, and/or cutaneous symptoms.

Physical and chemical characterization and pollution index applied in the assessment of the polluting potential of leachate from urban landfills.

During the operation of the landfills, leachate should be managed with caution to avoid possible negative environmental impacts. Considering this, the present study aims to evaluate the relationship between different variables in the leachate composition and elucidate the transformation processes through which this effluent passes during the landfill's period of operation. The study was conducted with eight sanitary landfills from the state of Minas Gerais, in southeastern Brazil, and used descriptive statistical analysis, principal component analysis (PCA), correlation analysis, and calculation of the leachate pollution index (LPI).

Treatment outcome of oropharyngeal squamous cell carcinoma through propensity score analysis.

Unlabelled: According to an extensive database, the Objective is to compare surgical versus non-surgical treatment through Propensity Score (PS) for patients with Oropharyngeal Squamous Cell Carcinoma (OPSCC).

Methods: We retrospectively evaluated epidemiological data from 8075 patients with OPSCC diagnosed between 2004 and 2014 and used PS matching to analyze possible prognostic factors for its outcomes with regression analyses.

Results: Cox multiple regression analysis to study survival after PS matching shows that type of treatment was associated with death with a hazard ratio of 1.

Assessing the role of protected areas in the land-use change dynamics of a biodiversity hotspot.

Although protected areas (PAs) are designed to safeguard natural ecosystems from anthropic modifications, many PAs worldwide are subjected to numerous human-induced impacts. We evaluated whether the establishment of PAs in the Upper Paraná River floodplain region could reduce anthropic landscape changes and whether there is a difference in protection when using different PA restriction categories. We analyzed the overall landscape dynamics using 30 years of land-use time series data and evaluated the change intensity via a partial land-use intensity analysis.

Predictive models of newborn body composition: a systematic review.

Objective: To analyze the prediction models of fat-free mass and fat mass of neonates who had air displacement plethysmography as a reference test.

Data Source: A systematic review of studies identified in the PubMed, Virtual Health Library (BVS), SciELO, and ScienceDirect databases was carried out. The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) checklist was used for inclusion of studies, the Transparent Reporting of a Multivariable Prediction Model for Individual Prognosis or Diagnosis (TRIPOD) report was used to select only predictive models studies, and the Prediction Model Risk of Bias Assessment Tool (PROBAST) was used to assess the risk of bias in the models.

[Autochthonous Rickettsia parkeri rickettsiosis in the Paranaense Forest, Misiones, Argentina].

We report an autochthonous case of Rickettsia parkeri rickettsiosis occurred in June 2018 in a forested area of the Urugua-í Provincial Park, Misiones, Argentina. No previous records of this disease in humans have been previously reported in this region. The epidemiological, ecological, clinical, and laboratory features required for a proper diagnosis and adequate treatment are described here.

Size does not matter: molecular phylogeny reveals one of the largest trematodes from vertebrates, the enigmatic , as a species of (Trematoda: Clinostomidae).

Despite the recent advances raised in the molecular era to the taxonomic knowledge of species of the family Clinostomidae, especially those belonging to the specious genus , some groups of these vertebrate parasites remain poorly studied. This is the case of species of the enigmatic genus Witenberg, 1926, until recently monotypic and restricted to South America, but with its occurrence expanded to North America after the description of . Rosser et al.

Prognostic factors in oropharyngeal squamous cell carcinoma in the state of São Paulo, Brazil: 10-year follow-up.

Objective: To descriptively analyze the epidemiological data, clinical stage, and outcomes of oropharyngeal squamous cell carcinoma in the state of São Paulo, Brazil, and to estimate the influence of clinical stage and treatment type on overall and disease-free survival.

Methods: We retrospectively analyzed epidemiological data from the São Paulo Cancer Center Foundation database relative to patients with oropharyngeal squamous cell carcinoma diagnosed between 2004 and 2014 in the state of São Paulo. Univariate and multivariate Cox regression analyses were performed to assess factors associated with the outcomes.