Overlap Intensity: An ImageJ Macro for Analyzing the HIV-1 In Situ Uncoating Assay.

Capsid uncoating is at the crossroads of early steps in HIV-1 replication. In recent years, the development of novel assays has expanded how HIV-1 uncoating can be studied. In the in situ uncoating assay, dual fluorescently labelled virus allows for the identification of fused viral cores.