Effects of beta-adrenergic blockade on blood flow distribution during hypoxaemia in fetal sheep.

Hypoxaemia in fetal sheep causes a decrease in vascular resistance of the heart, brain and adrenal gland which results in increased blood flow to these organs. Placental blood flow is maintained. To investigate whether increased beta-adrenergic activity during hypoxaemia is involved in these changes, the effects of propranolol on organ blood flows (using the microsphere method) and other cardiovascular variables were studied during fetal hypoxaemia (50% reduction of fetal haemoglobin saturation) in 5 chronically catheterized fetal sheep at 126 to 130 days of gestation.

Comparison of glucose and a glucose polymer for testing oral carbohydrate tolerance in pregnancy.

Forty-six pregnant patients with potential diabetes were studied to compare the use of glucose and a glucose polymer for carbohydrate tolerance testing. The first 26 patients had a glucose tolerance test and a glucose polymer tolerance test in randomized order an average of one week apart. The mean tolerance curves and insulin curves were similar for both agents.

Removal of a terminator structure by RNA processing regulates int gene expression.

The int gene of phage lambda encodes a protein involved in site-specific recombination. Its expression is regulated differentially during successive phases of the lambda infective cycle. The gene is transcribed early after infection from one promoter, pL, and later from a second promoter pI.

Transcription terminator involved in the expression of the int gene of phage lambda.

The phage lambda int gene is transcribed from two different promoters, pI and pL. Transcription from pI results in efficient synthesis of Int protein whereas transcription originating from pL results in poor int expression. The differential expression of Int from these two transcripts is dependent upon a site (sib) located distal to the int gene [Guarneros and Galindo, Virology 95 (1979) 119-126; Guarneros et al.

Analysis of two potential shuttle vectors containing herpes simplex virus defective DNA.

Two potential shuttle vectors which contained the identical herpes simplex virus type 1 (HSV-1) defective particle DNA (dDNA), but prokaryotic DNA of different origins, were examined for their stability when propagated in eukaryotic cells, and for their efficiency as shuttle vectors. Each chimeric molecule contained a 9.5 kilobase-pair (kb) EcoRI fragment (HSV12-7) representing a single unit of a class I HSV-1 dDNA.

Transcription of the sulA gene and repression by LexA.

The Escherichia coli sulA gene product is a highly unstable protein, whose synthesis in response to DNA damage is associated with an inhibition of septation. Genetic evidence as well as sequence information suggests that the sulA gene is part of the E. coli SOS system and is induced after DNA damage.

Fructosamine in diabetic pregnancy.

Fructosamine, an indicator of glycosylated serum protein, was measured in 79 non-diabetic pregnant women and 20 women with gestational diabetes. The test provided a clear discrimination between groups; it detected 17 (85%) of the women with gestational diabetes and gave only 4 (5%) false-positive results. 19 women with established diabetes before pregnancy had very high levels.

High-level expression in Escherichia coli of the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc protein.

A plasmid, pJL6, was constructed that contains a unique ClaI site twelve codons beyond the bacteriophage lambda cII gene initiation codon. This site allowed us to fuse the carboxy-terminal sequences of the avian myelocytomatosis virus (MC29) v-myc gene to the amino-terminal portion of the cII gene. Transcription of the hybrid gene is controlled from the phage lambda pL promoter.

Deletion analysis of the retroregulatory site for the lambda int gene.

The lambda int gene product, integrase, recombines phage and bacterial DNA at a specific site during the integration step of lysogeny. Regulation of integrase synthesis is complex. (1) Transcription of the gene can occur from either of two promoters.

High-level expression of oncogenes in Escherichia coli.

A plasmid, pJL6, was constructed that contains a unique Cla I site 12 codons beyond the bacteriophage lambda cII gene initiation codon, as well as an adjacent unique Hind III site. These sites allowed us to fuse the sequences from the avian myelocytomatosis virus (MC29) v-myc gene, the avian myeloblastosis virus (AMV) v-myb gene, and the Harvey murine sarcoma virus (Ha-MuSV) v-ras gene to the amino-terminal portion of the cII gene. Transcription of the hybrid genes is controlled from the lambda PL promoter.

Posttranscriptional control of bacteriophage lambda gene expression from a site distal to the gene.

The bacteriophage lambda int gene product, integrase, recombines the phage DNA with the host DNA at specific sites on each to accomplish lysogeny. The int gene is transcribed from two promoters, PL and PI, each regulated positively by lambda proteins. The expression of integrase is also controlled from a site, sib, in the b region of the phage genome.

Promoter for the establishment of repressor synthesis in bacteriophage lambda.

Transcription of the lambda repressor gene (cI) is positively regulated by the phage-encoded proteins cII and cIII. We have isolated and characterized the 5'-terminal region of this RNA and shown that it originates at a promoter (pE) located between genes cro and cII. The DNA sequence of this promoter shows little homology to other known promoters.