Publications by authors named "Coulter Q Ralston"
Article Synopsis
- The study investigates the cell types and states in the distal region of the uterine tube, which is linked to high-grade serous carcinoma (HGSC), a serious form of ovarian cancer.
- Researchers found that stem/progenitor cells (Slc1a3+) can differentiate into other cell types and that disruption of certain genes (Trp53 and Rb1) can prevent the transformation of these cells into cancer.
- It was revealed that pre-ciliated cells (specific marker expressions) are particularly vulnerable to becoming cancerous, suggesting they could be important targets for diagnosis and treatment.
Development of in vitro, preclinical cancer models that contain cell-driven microenvironments remains a challenge. Engineering of millimeter-scale, in vitro tumor models with spatially distinct regions that can be independently assessed to study tumor microenvironments has been limited. Here, we report the use of porous silk scaffolds to generate a high cell density neuroblastoma (NB) model that can spatially recapitulate changes resulting from cell and diffusion driven changes.
View Article and Find Full Text PDFDevelopment of novel therapeutics is limited by a lack of accurate preclinical models for testing, specifically the inability of traditional 2D culture (monolayer) to accurately mimic in vivo tumors. In this work, lyophilized silk fibroin scaffolds were used to develop 3D neuroblastoma models (scaffolded NB) using multiple neuroblastoma cell lines (SK-N-AS, KELLY, and SH-SY5Y). Cells grown on scaffolds in low (1%) and ambient (21%) oxygen were compared to traditional monolayer cell culture.
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