Publications by authors named "Corrine Whitby"
Article Synopsis
- Real Time Polymerase Chain Reaction (q-PCR) is a technique used in microbial ecology to measure gene quantities in environmental samples, including quantifying gene transcripts with reverse transcription.
- This method utilizes fluorescent chemistries that detect PCR amplicons during the reaction's exponential growth phase, avoiding biases found in 'end-point' PCR.
- The document provides a detailed protocol for extracting DNA and RNA from coastal sediments, along with guidelines for quantifying bacterial 16S rRNA genes and transcripts using q-PCR and RT-q-PCR, including how to create standard curves for accurate measurements.
