A new screening test has been developed using an enzyme-linked immunosorbent assay (ELISA) to detect various anabolic steroid metabolites in horse urine, addressing concerns of steroid misuse in horse racing.
The test specifically focused on 16beta-hydroxymestanolone and was created by raising polyclonal antibodies in sheep, showing strong cross-reactivity with various related steroid compounds.
Analysis of horse urine samples using this assay successfully identified metabolites from steroid treatments, demonstrating the effectiveness of these ELISA methods as initial screening tools for both new and known anabolic steroids.