In vitro production of cat-restricted Toxoplasma pre-sexual stages.

Sexual reproduction of Toxoplasma gondii, confined to the felid gut, remains largely uncharted owing to ethical concerns regarding the use of cats as model organisms. Chromatin modifiers dictate the developmental fate of the parasite during its multistage life cycle, but their targeting to stage-specific cistromes is poorly described. Here we found that the transcription factors AP2XII-1 and AP2XI-2 operate during the tachyzoite stage, a hallmark of acute toxoplasmosis, to silence genes necessary for merozoites, a developmental stage critical for subsequent sexual commitment and transmission to the next host, including humans.

Meek Micro-Skin Grafting and Acellular Dermal Matrix in Pediatric Patients: A Novel Approach to Massive Extravasation Injury.

(1) Background: Extravasation injuries in pediatric patients can lead to significant harm if they are not promptly diagnosed and treated. However, evidence-based standardization on extravasation management remains limited, particularly for extensive wound necrosis. This case report presents the management of an 8-week-old premature patient with an extensive extravasation injury involving the right forearm and dorsum of the hand.

Tubulin tyrosination regulates synaptic function and is disrupted in Alzheimer's disease.

Microtubules play fundamental roles in the maintenance of neuronal processes and in synaptic function and plasticity. While dynamic microtubules are mainly composed of tyrosinated tubulin, long-lived microtubules contain detyrosinated tubulin, suggesting that the tubulin tyrosination/detyrosination cycle is a key player in the maintenance of microtubule dynamics and neuronal homeostasis, conditions that go awry in neurodegenerative diseases. In the tyrosination/detyrosination cycle, the C-terminal tyrosine of α-tubulin is removed by tubulin carboxypeptidases and re-added by tubulin tyrosine ligase (TTL).

A plant-like mechanism coupling m6A reading to polyadenylation safeguards transcriptome integrity and developmental gene partitioning in .

Correct 3'end processing of mRNAs is one of the regulatory cornerstones of gene expression. In a parasite that must adapt to the regulatory requirements of its multi-host life style, there is a need to adopt additional means to partition the distinct transcriptional signatures of the closely and tandemly arranged stage-specific genes. In this study, we report our findings in of an m6A-dependent 3'end polyadenylation serving as a transcriptional barrier at these .

Workplace noise exposure and audiometric thresholds in dental technicians.

Noise is a well-known risk factor in occupational medicine. Several studies have been performed in workplaces with noise sources, especially in the industrial field; on the contrary, only a few studies have been carried to evaluate the noise exposure effects in non-industrial workplaces such as small factories, handicraft laboratories, and dental laboratories. The aims of this study were to evaluate workplace noise exposure and hearing thresholds in dental technicians.

Association between waste management and HBV among solid municipal waste workers: a systematic review and meta-analysis of observational studies.

Aim: To conduct a systematic review of this relationship using available published observational studies in the field of solid municipal waste treatment.

Methods: The review of the scientific literature was based on Medline and Scopus databases up to December 2012, using the keywords HBV, waste, solid, treatment, workers, disposal, and refuse in different combinations.

Results: 160 studies were found and checked.

[Risk of non-coherent optical radiation in the operating room].

In this study, we evaluated the exposure of operating room personnel to non-coherent optical radiation by measuring the emission of blue light generated by operating lights. The values we obtained were largely below the exposure limit value of 100 W x m(-2) x sr(-1) provided by Legislative Decree no. 81/08 and European Directive 2006/25/EC, showing how workers can be exposed for longer than 8 hours without risk of acute health effects, confirming what was already said in literature.

[Alcohol use in the construction industry: results of a survey].

The CAGE questionnaire, integrated with socio-demographic data, was submitted to 239 workers of two construction companies. The statistical analysis was performed using SPSS 17.0.

Biological risk and occupational health.

Many work activities include hazards to workers, and among these biological risk is particularly important, mostly because of different types of exposure, contact with highly dangerous agents, lack of limit values able to compare all exposures, presence of workers with defective immune systems and therefore more susceptible to the risk. Bioaerosols and dust are considered important vehicles of microganisms at workplaces and interaction with other occupational agents is assumed. Moreover, biological risk can be significant in countries with increasing economic development or particular habits and some biological agents are also classified as carcinogenic to human.

Occupational biological risk knowledge and perception: results from a large survey in Rome, Italy.

Background: A cross-sectional survey on knowledge and perception of occupational biological risk among workers in several occupations was carried out in the industrial area of Rome.

Methods: The study was carried out in the period of March-April 2010 using a questionnaire with 33 items on the following areas: a) socio-demographic data; b) perception of the biological risks in ordinary occupational activity; c) knowledge about biological risks; d) biological risks in the working environment. The questionnaire was submitted to a convenience sample of workers of an industrial area in Southern Rome.

[Management, quality of health and occupational safety and hospital organization: is integration possible?].

The evolution of the national and European legislation has progressively transformed the working environments into organized environments. Specific models for its management are being proposed, which should be integrated into general management strategies. In the case of hospitals this integration should consider the peculiar organizational complexity, where the management of the occupational risk needs to be integrated with clinical risk management and economic risk management.

[The history of radioactivity and its applications in hygiene and therapy].

Since Antiquity, the observation of matter and its composition has been fascinating for the human mind. It represented the core of philosophical studies since the atomistic theory by Leucippus and Democritus, as well as of the opposed theory of elements by Empedocles, Plato and Aristoteles. Research on the atom, on its spontaneous or artificial disgregation, on the practical application of radioactive substances has strongly influenced daily life and the development of knowledge.

[Assessment of the risk of arm repetitive movements among workers in the motor vehicle glass finishing industry].

The aim of the study was to evaluate risk associated with biomechanical overload of the upper limbs in workers exposed to repetitive movements, employed to the finishing of glasses for motor vehicles. The risk assessment was performed using the OCRA method (OCRA index for every worker and Check-List OCRA for every workstation) and the results have been distributed for exposure levels. Altogether the results suggested the existence of risk associated with repetitive movements of the upper limbs and different risk classes (high, medium, light, very light).

[Noise exposure in the coffee industry in the light of current community standards].

And' is studied the noise exposure (with the calculation of the Lep,d) and the auditory function (with the method MPB 2002) in employed to the coffee's workmanship. The purpose was double. To deepen aspects a little studied thin to today and to compare the results with the "values limit of exposure" and the "values of exposure that make to go off the action" foreseen by the European directive 2003 of February 6th 2003.

[Accidents among ambulance employees: epidemiologic features].

The accidents have been esteemed that are verified in four years in a group of 824 employees. The study has underlined a meaningful percentage (30.1%) of road accidents.

Respiratory allergies on the staff of the police force.

The aim of the study was to evaluate respiratory allergies of an occupational nature in the staff of the police force. Five hundred and eighty-five subjects were submitted for lung function tests by dynamic spirometry. Subjects with a history of respiratory symptoms and border line respiratory function underwent bronchial provocation tests with methacholine.

[Audiometry in the cellulose industry].

A noise level dosimetry and audiometric testing were conducted in a cellulose factory to determine the hazardous noise level and the prevalence of noise induced hearing loss among the exposed workers. The noise level was recorded up to 90 db (A) in several working areas. 18 workers, potentially exposed to noise injury, evidenced a significant hearing loss.

[Fanconi's anemia: in vitro tests for the individualization of heterozygotes].

Fanconi's anaemia (FA) is an autosomal recessive mutation associated with constitutional chromosome aberrations. Patient's cells show increased susceptibility to different mutagens, especially bifunctional alkylating agents. Asymptomatic heterozygotes have a population prevalence of 1 in 300, and have been considered at risk for cancers.