Controlled Dimerization of Mn Single-Molecule Magnets.

Controlled dimerization of Mn single-molecule magnets (SMMs) was achieved via a synthetic route involving a competition between bridging and terminal ligands, namely, diols and alcohols. The reaction using a 1:1 ratio of the competing ligands resulted in the isolation of a new family of covalently linked dimers of Mn SMMs. This is the first step toward the controlled growth of SMM oligomeric arrays.

Introducing Dimensionality to the Archetypical Mn12 Single-Molecule Magnet: a Family of [Mn12]n Chains.

The [Mn12O12(O2CR)16(L4)] family (R = various; L = terminal ligand) of clusters holds a special place in molecular magnetism; they are the most well-studied single-molecule magnets (SMMs). Targeted linkage of these SMMs has now been achieved for the first time. The resulting chain structures have been confirmed crystallographically, and the magnetic properties, up to 1.

Characterization of a family of ice-active proteins from the Ryegrass, Lolium perenne.

Five genes coding for ice-active proteins were identified from an expressed sequence tag database of Lolium perenne cDNA libraries. Each of the five genes were characterized by the presence of an N-terminal signal peptide, a region enriched in hydrophilic amino acids and a leucine-rich region in four of the five genes that is homologous with the receptor domain of receptor-like protein kinases of plants. The C-terminal region of all five genes contains sequence homologous with Lolium and Triticum ice-active proteins.

Influence of estradiol and gestagens on oxidative stress in the rat uterus.

Objective: We studied the effect of ovariectomy, estradiol (E2), and E2 + medroxyprogesterone (MPA) on the Wistar rat uterus.

Methods: We used 15 adult female rats. The study was divided into the following four stages: (a) extirpation of the upper half of the left hemi-uterus (basal state) and ovariectomy; (b) animals were maintained for 15 days without treatment, performance of a new laparotomy, and extirpation of the remaining left hemi-uterus (OVX state); (c) beginning of E2 replacement therapy (ERT) (8 microg/day) for 15 days, followed by extirpation of the upper half of the right hemi-uterus (ERT state); and (d) the administration of E2 was continued, and oral treatment with MPA was begun (20 microg/day) to last for a further 15 days.

Folding kinetics of phage 434 Cro protein.

Folding kinetics for phage 434 Cro protein are examined and compared with those reported for lambda(6-85), the N-terminal domain of the repressor of phage lambda. The two proteins have similar all-helical structures consisting of five helices but different stabilities. In contrast to lambda(6-85), sharp and distinct aromatic (1)H NMR signals without exchange broadening characterize the native and urea-denatured 434 Cro forms at equilibrium at 20 degrees C, indicating slow interconversion on the NMR time scale.

Reversible denaturation of myo-inositol monophosphatase. The stability of the metal-binding loop.

The unfolding of bovine brain myo-inositol monophosphatase by guanidine. HCl (Gdn. HCl) has been investigated.

Study of the interaction between xylazine and bovine serum albumin by fluorescence quenching measurements.

The binding of xylazine to bovine werum albumin (BSA) was studied by fluoresence quenching, as a function of temperature. The experimental data could be fitted to both the Stern-Volmer equation and the Stern-Volmer equation modified by Lehrer. The temperature dependence of the Stern-Volmer constant, Ksv suggests that the mechanism of the quenching process is mainly dynamic in origin.