Publications by authors named "Cornelia Hiemenz"
Genome length determination in adeno-associated virus vectors with mass photometry.
Mol Ther Methods Clin Dev
December 2023
Recombinant adeno-associated viruses (rAAVs) are attractive therapeutic viral vectors for gene delivery. To ensure the efficacy and safety of rAAV-based therapies, comprehensive characterization of the adeno-associated virus (AAV) capsids is essential. Mass photometry (MP) provides the advantage of short analysis times, low sample consumption, and high accuracy of molecular mass determination.
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- Characterizing impurities like aggregates in adeno-associated virus (AAV) products is crucial for ensuring their safety and effectiveness.
- The study examined three techniques—mass photometry (MP), asymmetric flow field flow fractionation with UV detection (AF4-UV/Vis), and microfluidic resistive pulse sensing (MRPS)—to analyze AAV monomers and aggregates under 1 µm in size.
- While MP proved to be a quick and accurate method for quantifying genome content in various capsid types, AF4-UV/Vis effectively separated monomers from small aggregates, and MRPS was useful for measuring particle size and concentration, highlighting the strengths and weaknesses of each method in studying AAV aggregates.