A strategy to establish a gene-activated matrix on titanium using gene vectors protected in a polylactide coating.

Bioactive implants are promising tools in regenerative medicine. Here we describe a versatile procedure for preparing a gene-activated matrix on titanium. Lyophilized copolymer-protected gene vectors (COPROGs) suspended in poly(d,l-lactide) (PDLLA) solutions in ethyl acetate were used to varnish solid surfaces.

YB-1 dependent virotherapy in combination with temozolomide as a multimodal therapy approach to eradicate malignant glioma.

The human Y-box binding protein 1 (YB-1) is known to be a promising target for cancer therapy. We have demonstrated that YB-1 plays an important role in the adenoviral life cycle by regulating the adenoviral E2-gene expression. Thus, we studied the oncolytic effect of the recombinant adenovirus Ad-Delo3-RGD, in which the transactivation domain CR3 of the E1A protein is ablated to enable viral replication only in YB-1 positive cancer cells.

Characterization of eight different tetracyclines: advances in fluorescence bone labeling.

Polychrome sequential labeling with fluorochromes is a standard technique for the investigation of bone formation and regeneration processes in vivo. However, for human application, only tetracycline and its derivates are approved as fluorochromes. Therefore, the aim of this study was to determine the fluorescence characteristics of the different tetracycline derivates to assess the feasibility of sequential in vivo bone labeling using distinguishable fluorochromes.

Laser-modified titanium implants for improved cell adhesion.

Concerning dental implant systems, a main problem is the adhesion of peri-implant mucosa in the cervical region. The aim of the present study was to use a laser for modifying titanium implants to promote mucosal adhesion, which is indispensable as a biological barrier against bacterial infection. By the use of a KrF excimer laser, it was possible to induce a holey structure on the polished area of the implant surface, which was analysed by a scanning electron microscope.

Effect of 308-nm excimer laser light on peri-implantitis-associated bacteria: an in vitro investigation.

Dental implants are becoming increasingly important in prosthodontic rehabilitation. Bacterial infections, however, can induce bone loss and jeopardize clinical success. Recent literature has demonstrated that infrared CO(2) laser light is suitable for the decontamination of exposed implant surfaces.

New advances in fluorochrome sequential labelling of teeth using seven different fluorochromes and spectral image analysis.

Fluorochrome sequential labelling of mineralizing tissues is commonly used in different fields of clinical and basic research. Recently we improved polychrome fluorescent sequential labelling of bone by applying spectral image analysis to discriminate seven different fluorochromes. Although basic mineralization processes of bone and teeth follow comparable principles, the respective tissues differ in terms of matrix composition and mineral assembly.