Publications by authors named "Corinne S V Geewan"
J Membr Biol
October 2011
Article Synopsis
- NKCC1 is a crucial membrane transporter in chondrocytes, impacting volume regulation, matrix synthesis, and bone growth, but its regulation is not well understood.
- A knockdown technique using siRNA was developed to specifically reduce NKCC1 expression in the C-20/A4 chondrocyte cell line, achieving a reduction to about 25% after 12 hours, confirmed by various analytical methods.
- The study showed that NKCC1 knockdown led to a slower regulatory volume increase (RVI) response in chondrocytes after exposure to hypertonic conditions, highlighting NKCC1's role in cell volume maintenance.
