Publications by authors named "Corentin Gondrand"
Article Synopsis
- Mitochondrial NAD acts as a key electron transporter and co-factor for various enzymatic reactions, particularly in ADP-ribosylation, though its specific role in mitochondria is not well understood.
- Research reveals that mitochondrial ADP-ribosylation increases when the respiratory chain is inhibited and decreases under oxidative stress, suggesting a reversible response to cellular conditions.
- The study suggests a dynamic relationship between mitochondrial and nuclear ADP-ribosylation, where changes in mitochondrial ADP-ribosylation influence nuclear processes and highlight a potential NAD-mediated communication pathway between the two.
The cellular level of nicotinamide adenine dinucleotide (NAD), through its different functions, affects cellular metabolism and signalling. A decrease in the NAD content has been associated with various pathologies and physiological aging, while strategies to boost cellular NAD levels have been shown to be effective against age-related diseases in many animal models. The link between decreased NAD levels and numerous pathologies and physiological aging has triggered the need for a simple quantification method for NAD, ideally applicable at the point of care.
View Article and Find Full Text PDFWe introduce a new class of semisynthetic fluorescent biosensors for the quantification of free nicotinamide adenine dinucleotide (NAD) and ratios of reduced to oxidized nicotinamide adenine dinucleotide phosphate (NADPH/NADP) in live cells. Sensing is based on controlling the spatial proximity of two synthetic fluorophores by binding of NAD(P) to the protein component of the sensor. The sensors possess a large dynamic range, can be excited at long wavelengths, are pH-insensitive, have tunable response range and can be localized in different organelles.
View Article and Find Full Text PDF