An update on granulomatous mastitis: a rare and complex condition.

Granulomatous mastitis is a rare inflammatory condition of the breast affecting women of child-bearing age. The disease is characterised by chronic and relapsing inflammation, resulting in scarring and discharging sinuses. Granulomatous mastitis is considered to be idiopathic and the pathogenesis is poorly understood.

Survival outcomes following aggressive treatment of oligometastatic breast cancer: a systematic review protocol.

The objective of this systematic review is to assess the effectiveness of aggressive treatment of oligometastatic breast cancer (OMBC) on survival outcomes by conducting a meta-analysis of current available evidence.More specifically, the objectives are to identify the effectiveness of intensified multidisciplinary treatment with aggressive locoregional therapies on survival time, five-year survival rates and disease free survival. The population is adult women (18 years and over) with OMBC defined as single or few (five or less) metastases limited to a single organ and the comparative group is conventional palliative treatment aimed at disease control.

Comorbidity assessment in localized prostate cancer: a systematic review protocol.

The objective of this study is to review and summarize the methods and tools used to measure comorbidity in localized prostate cancer (PCa) and in particular to assess whether these tools are adequately validated and reliable for determining the impact of comorbidity on survival and treatment decisions for this disease.Specifically, the review questions are.

Histopathological evaluation of parotid gland neoplasms in Queensland, Australia.

Background: Parotid gland tumours are complex neoplasms with a broad histological range. The parotid gland is also a common site of face and scalp skin cancer metastases.

Method: Parotidectomies performed by ENT department in the Gold Coast health district from 2006 to 2013.

Aminoglycoside-modifying enzymes associated with hospital isolates of Gram-negative rods.

Multi-resistant Enterobacteriaceae and Pseudomonas isolated from clinical specimens at RNS were characterized for resistance phenotype and transferred resistant phenotype. These isolates were obtained over a two year period from different specimens and from patients with varying clinical syndromes. Twenty-six of these isolates were further characterized with respect to their aminoglycoside modifying enzymes.

Purification and properties of an aminoglycoside acetyltransferase from Pseudomonas aeruginosa.

An aminoglycoside 3-acetyltransferase [AAC(3)], possibly a new isoenzymic species of the 3-N-acetyltransferase group, was purified to apparent homogeneity from a crude extract of Pseudomonas aeruginosa, a gentamicin-resistant clinical isolate. The method of purification was consecutive column chromatography--(i) gel filtration, (ii) affinity chromatography, and (iii) ion-exchange chromatography--to give two protein peaks, one of which was coincident with activity and which indicated a purification of 600 (specific activity = 9.743 units mg-1 at pH 7.

New plasmid-mediated aminoglycoside adenylyltransferase of broad substrate range that adenylylates amikacin.

The same aminoglycoside 2"-adenylyltransferase was isolated from four gram-negative species which were among a random group of gentamicin-resistant isolates from the same hospital. The enzyme was partially purified from a crude extract which also contained a second modifying enzyme identified as APH(3')-I. The substrate range of the new aminoglycoside 2"-adenylyltransferase included the newer aminoglycosides sisomicin and amikacin, but showed much-reduced activity against gentamicins C2 and Cla.

An alternative coupling procedure for preparing activated sepharose for affinity chromatography of penicillinase.

Most applications of affinity chromatography employ the cyanogen bromide activation scheme first devised by Axèn et al. (1967). Porath and Sunberg (1972) reported an alternative procedure in which phloroglucinol and divinylsulphone are used in activating reactions.