Crude preparations of Dioon spinulosum dyer neurotoxicity: methylazoxymethanol produces petil mal seizures in susceptible individuals.

Dioon spinulosum is a cycad prepared as an alternative food and consumed by Chinantecos from Veracruz and Oaxaca, México. Whether Dioon spinulosum possesses neurotoxic properties is unknown, therefore, we used wistar rats receiving several regimens of D. spinulosum.

Raphe-septal neurons changes in sensitivity to desipramine following an early septal lesion in the rat.

1. The electrophysiological responsivity to desipramine (DMI) applied by systemic or local route was tested in Wistar female rats submitted to a wide lesion in the lateral septal area on the 8th day after birth. 2.

Microculture in biphasic medium with silicone-coated slides for isolation of mycobacteria.

The study reported here, seeking to develop a simple, practical, sensitive, and inexpensive technique for microbial diagnosis of tuberculosis, used a combination of biphasic media and microculture techniques to augment the sensitivity of traditional culture methods. A total of 540 sputum samples (5 mL each) were obtained from 180 patients with suspected tuberculosis in Mexico City. These samples were treated with Hanks reagent, neutralized with 25% HCl, and centrifuged.

Diuretic and natriuretic effects of chromatographically purified fraction of garlic (Allium sativum).

The intravenous administration of chromatographically purified fractions of garlic (2, 4 and 6 micrograms/kg dry weight) to anaesthetized rabbits elicits dose-dependent diuretic-natriuretic responses which reach a maximum 60 min after injection, and return to basal levels after 90 min. A gradual decrease in heart rate, but not in arterial blood pressure was observed during the course of the experimental periods. The electrocardiogram was not affected.

Anticonvulsant properties of Ipomoea stans.

Ipomoea stans (IS) has long been used as an anticonvulsant. Although the use of HPLC and nuclear magnetic resonance techniques recently have allowed some of its compounds to be identified, the anticonvulsant properties of this Convolvulaceae remained to be tested. During the course of several weeks, mice (BALB/C) were given a normal infusion of the plant instead of clean water (IS-ad lib).

An early lesion of the lateral septal nuclei produces changes in the forced swim test depending on gender.

1. Several pharmacological maneuvers in very young rats produce later changes resembling human depression. 2.

The combination of several antidepressants is not synergistic on the firing of lateral septal neurons in the rat.

1. Three kinds of antidepressants (clomipramine, sleep deprivation, and electroconvulsive shock) increase the firing rate in the lateral septal neurons of the rat. 2.

Electroconvulsive shock decreases excitatory responses to serotonin in the caudate nucleus of the rat.

1. The present study explored the changes on the firing rate of caudate neurons, and the response to serotonin locally applied in rats submitted to electroconvulsive shock. 2.

[The recommendable frequency for performing electrocardiograms in the hypertension patient].

Objective: To obtain information on how often electrocardiograms (ECG) should be carried out during hypertense patients' follow-up.

Design: A retrospective, descriptive study.

Setting: La Mina Primary Care Centre.

Clomipramine increases firing rate in lateral septal neurons related with hippocampus.

Long-term clomipramine administration (CMI: 2.5 mg/kg IP, 25 days) effects on 274 identified hippocampus-septal neurons was assessed in rats. Stimulation of CA 1/dentate areas produced a biphasic response in 61% of lateral septal neurons.

[Anxiety and depression levels in rape offenders].

Whether the active subject of rape possesses a particular personality trait is a much debated matter. It is an accepted fact that a small amount of rapists fall into the sadistic diagnostic category. Other psychopatologic traits likely to identify a rapist are, however, unknown.

Clomipramine enhances the excitatory actions of dorsal raphe nucleus stimulation in lateral septal neurons in the rat.

Clomipramine is a serotonin reuptake blocker which produces an increased rate of firing in lateral septal neurons of the rat. However, it is unknown whether the response of dorsal raphe nucleus stimulation on the firing rate of the lateral septal neurons is modified by clomipramine treatment. Two programs of stimulation were employed.

Sleep deprivation is a less potent agent than clomipramine in increasing firing rate in lateral septal neurons in the rat.

Acute injection of clomipramine or a 24-hour period of sleep deprivation produces an increased firing rate in lateral septal neurons of the rat. However, it is unknown whether changes in firing rate in lateral septal neurons also appear after repetitive treatments. This study explored and compared the effects of long-term clomipramine with those of sleep deprivation from 1 to 4 days in the firing rate of lateral septal neurons.

Clomipramine increases the responsiveness of raphe-cortical neurons in the rat.

The cerebral cortex of the rat possesses 5-HT1A receptor subtype; however, whether the activation of this receptor by dorsal raphe nucleus stimulation produces a similar action on neuronal cortical firing as antidepressants acting on serotonin (5-HT) function is unknown. Single-unit extracellular recordings were obtained from parietal cortex in rats in which dorsal raphe nucleus or cerebellar peduncle was stimulated. Results compared groups long-term treated with clomipramine (1.

[Major and minor changes in the electrocardiogram of the hypertensive patient].

Objective: To determine the prevalence and characteristics of electrocardiograph deviation in such hypertensive patients.

Design: Retrospective descriptive study of a representative sample of patients with high blood pressure. SITE.

Detection of human antibodies against Plasmodium falciparum antigens in blood meals of anopheline mosquitoes.

Human IgG antibodies against Plasmodium falciparum asexual stages, gametocytes and sporozoites were detected by indirect fluorescent antibody (IFA) techniques in the blood meals of Anopheles gambiae s.l. from a malaria-endemic area of western Kenya.

[Study of diffuse chronic hepatopathy in the outpatient context].

Objective: Description of the general characteristics of patients with Diffuse Chronic Hepatopathy (DCH) in an ABS.

Design: Empirical study of a prospective and retrospective type. Site.

Amygdalar catecholaminergic input to septal nuclei, relation to clomipramine actions on lateral septal neurons in the rat.

Antidepressants exert mixed actions on serotonergic and catecholaminergic systems. However, it is unknown whether a catecholaminergic blockade impinge on the actions of a tricyclic with serotonergic agonist properties (clomipramine) in limbic structures. The aim of the present study is to explore the effects of a catecholaminergic lesion in the basolateral amygdala on the firing rate of lateral septal, and hippocampal neurons in rats treated with clomipramine.

Cortical and septal responses to dorsal raphe nucleus stimulation in the rat: long-term clomipramine actions.

In cerebral cortex and lateral septal nuclei different serotonergic receptor subtypes coexist, thus a different action on neuronal firing may be expected depending on the receptor activated. Dorsal raphe nucleus stimulation produced an increased rate of firing in cortical layer V, and in lateral septal nuclei. However, firing rate in cortical layer VI remained unchanged after stimulating the dorsal raphe nucleus.

Septal neurons related with hippocampus increase their firing rate after long-term clomipramine.

In the rat, long-term clomipramine increases the firing rate in lateral septal neurons. Although the hippocampus is the main afference for septal nuclei, it is unknown whether clomipramine increases the firing rate in most of hippocampal-septal neurons. Therefore, the present study explored the actions of long-term clomipramine in lateral septal neurons identified by their relation to the hippocampus.