Publications by authors named "Constanza Pautasso"
Article Synopsis
- cAMP regulates various cellular processes in yeast (Saccharomyces cerevisiae) through a protein called PKA, which is crucial for responding to heat shock and adapting to thermal stress.
- The study shows that the mRNA for the TPK1 gene, part of the PKA holoenzyme, increases in abundance and stability during heat shock, but its translation is inhibited.
- Additionally, the presence of the Wsc3 sensor and some components of the CWI pathway are essential for the expression of TPK1 during heat stress, indicating that thermal stress influences the specificity of cAMP-PKA signaling and enhances yeast's ability to cope with heat.
Chromatin remodeling and transcription of the TPK1 subunit of PKA during stress in Saccharomyces cerevisiae.
Biochim Biophys Acta Gene Regul Mech
September 2020
In response to environmental changes cells rapidly rearrange their gene expression pattern in order to adapt to the new conditions. Chromatin remodeling is critical for this process playing a major role in the induction of genes involved in stress responses. We demonstrated previously that TPK1, encoding one of the catalytic subunits of PKA from Saccharomyces cerevisiae, is upregulated under heat shock.
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- Yeast cells, specifically Saccharomyces cerevisiae, prefer glucose for growth and switch to fermentative metabolism even when oxygen is available, undergoing a diauxic shift when glucose is depleted.
- When glucose is present, a regulatory mechanism called glucose repression affects gene expression, leading to changes in how yeast utilize other carbon sources during glucose derepression.
- The study explores the regulation of PKA subunit promoters during different metabolic states, finding that while all promoters are upregulated with glycerol, only the TPK1 subunit is repressed by a specific protein complex when glucose is present.
The cAMP-dependent protein kinase (PKA) signaling is a broad pathway that plays important roles in the transduction of environmental signals triggering precise physiological responses. However, how PKA achieves the cAMP-signal transduction specificity is still in study. The regulation of expression of subunits of PKA should contribute to the signal specificity.
View Article and Find Full Text PDFProtein kinase A (PKA) is a broad specificity protein kinase that controls a physiological response following the increment of cAMP as a consequence of a particular stimulus. The specificity of cAMP-signal transduction is maintained by several levels of control acting all together. Herein we present the study of the regulation of the expression of each PKA subunit, analyzing the activity of their promoters.
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