Publications by authors named "Constantinos Chatzicharalampous"
Article Synopsis
- - Poly-ADP-ribose polymerase-14 (PARP14) is an enzyme that modifies proteins and nucleic acids, and its abnormal functions are linked to cancer, inflammation, and viral infections.
- - Researchers faced challenges in studying PARP14 due to difficulties in obtaining pure protein for in vitro experiments, which is crucial for understanding its functions.
- - The study introduces a new method for producing a functional PARP14 construct in bacteria, including a modified domain that maintains its enzymatic activities, enabling better structural and functional research on PARP14.
PARP14/BAL2 is a large multidomain enzyme involved in signaling pathways with relevance to cancer, inflammation, and infection. Inhibition of its mono-ADP-ribosylating PARP homology domain and its three ADP-ribosyl binding macro domains has been regarded as a potential means of therapeutic intervention. Macrodomains-2 and -3 are known to stably bind to ADP-ribosylated target proteins, but the function of macrodomain-1 has remained somewhat elusive.
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