The Outer Membrane β-Barrel Assembly Machinery (BAM) Crosstalks with the Divisome.

The BAM is a macromolecular machine responsible for the folding and the insertion of integral proteins into the outer membrane of diderm Gram-negative bacteria. In , it consists of a transmembrane β-barrel subunit, BamA, and four outer membrane lipoproteins (BamB-E). Using BAM-specific antibodies, in cells, the complex is shown to localize in the lateral wall in foci.

The Outer Membrane β-Barrel Assembly Machinery (BAM) Anchors the Peptidoglycan Layer by Spanning It with All Subunits.

Gram-negative bacteria possess a three-layered envelope composed of an inner membrane, surrounded by a peptidoglycan (PG) layer, enclosed by an outer membrane. The envelope ensures protection against diverse hostile milieus and offers an effective barrier against antibiotics. The layers are connected to each other through many protein interactions.

Polysulfides Applied as Formulated Garlic Extract to Protect Tomato Plants against the Root-Knot Nematode .

The devastating root-knot nematode can cause severe damage to field and greenhouse crops. Due to high economic losses, alternative products are essential to replace banned or strictly regulated nematicides that affect human health and/or the environment. Garlic based products have been previously investigated as environmentally friendly nematicides and their active substances, diallyl polysulfides exist as formulated nematicides on the market.

MreC and MreD balance the interaction between the elongasome proteins PBP2 and RodA.

Rod-shape of most bacteria is maintained by the elongasome, which mediates the synthesis and insertion of peptidoglycan into the cylindrical part of the cell wall. The elongasome contains several essential proteins, such as RodA, PBP2, and the MreBCD proteins, but how its activities are regulated remains poorly understood. Using E.

Detection of Protein Interactions in All Bacterial Components by Förster Resonance Energy Transfer with the Superfolder mTurquoise2 ox-mNeongreen FRET Pair.

This protocol was developed to qualitatively and quantitatively detect protein-protein interactions in all compartments of by Förster Resonance Energy Transfer (FRET) using the Superfolder mTurquoise2 ox-mNeonGreen FRET pair (sfTq2-mNG). This FRET pair has more than twice the detection range for FRET interaction studies in the cytoplasm or periplasm of compared to other pairs to date. These protein-interaction studies can be performed because fluorescent proteins can be genetically encoded as fusions to proteins of interest and expressed in the cell.

First Morphological and Molecular Report of on Strawberry Plants in Switzerland.

Foliar nematodes represent a minor feeding group within the genus Fischer, 1894. The facultative plant parasitic species can cause crinkling of leaves, reduced vigor, and stunting of agricultural and ornamental plants. Here we report the first finding of in leaves, crowns, and roots of strawberry plants collected in Switzerland in 2018.

Superfolder mTurquoise2 optimized for the bacterial periplasm allows high efficiency in vivo FRET of cell division antibiotic targets.

Fluorescent proteins (FPs) are of vital importance to biomedical research. Many of the currently available fluorescent proteins do not fluoresce when expressed in non-native environments, such as the bacterial periplasm. This strongly limits the options for applications that employ multiple FPs, such as multiplex imaging and Förster resonance energy transfer (FRET).

Proteomics-Based Tools for Evaluation of Cell-Free Protein Synthesis.

Cell-free protein synthesis (CFPS) has the potential to produce enzymes, therapeutic agents, and other proteins, while circumventing difficulties associated with in vivo heterologous expression. However, the contents of the cell-free extracts used to carry out synthesis are generally not characterized, which hampers progress toward enhancing yield or functional activity of the target protein. We explored the utility of mass spectrometry (MS)-based proteomics for characterizing the bacterial extracts used for transcribing and translating gene sequences into proteins as well as the products of CFPS reactions.

Molecular identification and phylogenetic analysis of Metarhabditis blumi (Nematoda: Rhabditida).

The species Metarhabditis blumi was diagnosed for the first time in Brazil using a molecular assay. Parasites isolated from two cattle herds from the state of Minas Gerais, southeastern Brazil, were classified as M. blumi according to their D2/D3 expansion fragments of the 28S rDNA sequence, which had a high degree of homology (100% similarity) with a M.

Use of luminescent gunshot residues markers in forensic context.

Chemical evaluation of gunshot residues (GSR) produced by non-toxic lead-free ammunition (NTA) has been a challenge to forensic analyses. Our group developed some luminescent markers specific to the detection of GSR. Here, we evaluated the performance of selected markers in experiments that mimic forensic context and/or routines in which luminescent characteristics would be very useful.

Diffusion of light-harvesting complex II in the thylakoid membranes.

The light-harvesting complex II (LHCII) is the main energy absorber for photosynthesis in green plants, and its translocation between photosystems I and II is the primary means of energy redistribution between them. Using single-particle tracking, we performed the first measurement of the mobility of LHCII in the photosynthetic membranes in both the nonphosphorylated and the phosphorylated (P-LHCII) conformations. These are part of an important, reversible, energy re-equilibration process called the state transition.