Treatment of a Rat Model of LPS-Induced ARDS via Peritoneal Perfusion of Oxygen Microbubbles.

Acute respiratory distress syndrome (ARDS) is a serious respiratory condition that occurs in approximately 10% of patients entering intensive care units around the world, affecting nearly 190,000 patients annually in the United States. Owing to the severity of the condition, conventional methods of oxygenation are often insufficient. However, current alternate methods of oxygenation are associated with contraindications and a mortality rate near 50%.

Plane-Wave Contrast Imaging: A Radiation Force Point of View.

Radiation force is known to produce microbubble axial displacements by an amount that depends on the transmit burst frequency, amplitude, and length, as well as the pulse repetition frequency (PRF). In the standard focused-imaging mode, the actual PRF experienced by each microbubble is low, because it is of the order of the frame rate (i.e.

Click Conjugation of Cloaked Peptide Ligands to Microbubbles.

Interest in the use of targeted microbubbles for ultrasound molecular imaging (USMI) has been growing in recent years as a safe and efficacious means of diagnosing tumor angiogenesis and assessing response to therapy. Of particular interest are cloaked microbubbles, which improve specificity by concealing the ligand from blood components until they reach the target vasculature, where the ligand can be transiently revealed for firm receptor-binding by ultrasound acoustic radiation force pulses. Herein, a bio-orthogonal "click" conjugation chemistry is introduced to decorate the surface of cloaked 4-5-μm-diameter microbubbles as part of a sterile and reproducible production process.