Detection of zearalenone by electrochemical aptasensor based on enzyme-assisted target recycling and DNAzyme release strategy.

Zearalenone has a high level of detection and exceedance in cereals and by-products. Herein, an electrochemical aptasensor for ZEN detection was proposed. The selected aptamer, which has a high affinity for ZEN, serves as a molecular recognition element and effectively avoids interference from other toxins.

A label-free ratiometric homogeneous electrochemical aptasensor based on dual catalytic hairpin self-assembly for rapid and sensitive detection of ochratoxin A in food.

The food contamination with Ochratoxin A (OTA) has highlighted the need to create precise, sensitive, and convenient techniques. Herein, we proposed a label-free and immobilization-free ratiometric homogeneous electrochemical aptasensor based on dual catalytic hairpin self-assembly (CHA) for OTA detection. Methylene blue (MB) and ferrocene (Fc) in solution were utilized as label-free signaling molecules, generating a response signal (I) and a reference signal (I), respectively.

A high molecular weight polymalate is synthesized by the whole genome duplicated strain Aureobasidium melanogenum OUC.

Polymalate (PMA) produced by the whole genome duplicated strain Aureobasidium melanogenum OUC had a high molecular weight (Mw) of 3.9 × 10 Da while the Mw of PMA produced by A. melanogenum ATCC62921 was 3.

Polymalate (PMA) biosynthesis and its molecular regulation in Aureobasidium spp.

It has been well documented that different strains of Aureobasidium spp. can synthesize and secrete over 30.0 g/L of polymalate (PMA) and the produced PMA has many potential applications in biomaterial, medical and food industries.

The differences between fungal α-glucan synthase determining pullulan synthesis and that controlling cell wall α-1,3 glucan synthesis.

The fungal α-glucan synthases (Agss) are multi-domain proteins catalyzing biosynthesis of cell wall α-1,3-glucan which determines cell wall integrity or fungal pathogenicity and pullulan which is a maltotriosyl polymer made of α-1,4 and α-1,6 bound glucose units. The Agss family can be divided into 11 groups, some of which lost the original functions due to accumulation of harmful mutations or gene loss. Schizosaccharomyces pombe kept five kinds of Agss in the genome while Aspergillus spp.

A novel PMA synthetase is the key enzyme for polymalate biosynthesis and its gene is regulated by a calcium signaling pathway in Aureobasidium melanogenum ATCC62921.

It has been well known that poly(β-l-malic acid)(PMA) has many potential applications. However, it is still completely unknown how PMA is biosynthesized in Aureobasidium spp. In this study, it was found that malic acid from TCA cycle was the main source for PMA biosynthesis.

[Characteristic analysis of acute leukemia immunophenotyping in 180 cases by flow cytometry].

To study the acute Leukemia immunophenotype and its diagnosis value, three color direct immunofluorescece staining methods of flow cytometry and immunophenotype of antibody integration system were used for detection of 180 cases of acute leukemia. The results showed that patients with ALL expressed lymphocyte antigen, and 49.4% patients with ALL accompanied myeloid antigen; all patients with AML expressed myeloid antigen, and 43.