Precise CRISPR/Cpf1 genome editing system in the Deinococcus radiodurans with superior DNA repair mechanisms.

Deinococcus radiodurans, with its high homologous recombination (HR) efficiency of double-stranded DNA breaks (DSBs), is a model organism for studying genome stability maintenance and an attractive microbe for industrial applications. Here, we developed an efficient CRISPR/Cpf1 genome editing system in D. radiodurans by evaluating and optimizing double-plasmid strategies and four Cas effector proteins from various organisms, which can precisely introduce different types of template-dependent mutagenesis without off-target toxicity.

Porous Graphene Produced by Carbothermal Shock for Green Electromagnetic Interference Shielding in Both Microwave and Terahertz Bands.

The prospect of graphene-based shielding materials in the form of fillers is limited by the cumbersome preparation of graphene. Herein, defect-tunable porous graphene prepared by carbothermal shock using low-value sucrose as a precursor is proposed as an effective shielding filler. The resultant porous graphene exhibits 32.

Probing the sORF-Encoded Peptides of Deinococcus radiodurans in Response to Extreme Stress.

Organisms have developed different mechanisms to respond to stresses. However, the roles of small ORF-encoded peptides (SEPs) in these regulatory systems remain elusive, which is partially because of the lack of comprehensive knowledge regarding these biomolecules. We chose the extremophile Deinococcus radiodurans R1 as a model species and conducted large-scale profiling of the SEPs related to the stress response.

Proteogenomic discovery of sORF-encoded peptides associated with bacterial virulence in Yersinia pestis.

Plague caused by Yersinia pestis is one of the deadliest diseases. However, many molecular mechanisms of bacterial virulence remain unclear. This study engaged in the discovery of small open reading frame (sORF)-encoded peptides (SEPs) in Y.

Diagnostic value of phosphatidylethanolamine binding protein 4 levels in patients receiving nursing interventions for advanced chronic kidney disease.

Objective: To explore the diagnostic role of phosphatidylethanolamine binding protein 4 (PEBP4) in patients with chronic kidney disease (CKD) receiving nursing interventions.

Methods: ELISA was used to evaluate serum PEBP4 levels. Receiver-operating characteristic curve analysis was used to assess diagnostic accuracy.

Cryopreservation of germline stem cells in American paddlefish (Polyodon spathula).

Most sturgeon and paddlefish are critically endangered; therefore, effective measures to conserve these genetic resources are required. Cryopreservation of gonad tissues containing germline stem cells could be an effective strategy for long term preservation and restoration of fish species using germ cell transplantation procedure. The aim of this study was to develop an optimal procedure for long-term cryopreservation of American paddlefish gonads using a slow-freezing method.

Assessment of Yangtze sturgeon as recipient for the production of American paddlefish gametes through spermatogonia transplantation.

The Chinese paddlefish (Psephurus gladius), one of the world's largest freshwater fish, was last seen alive in 2003; they are presumed now to be extinct. In fish, germ cell transplantation is currently known as one of the most powerful assisted reproductive technologies for the conservation of endangered species. In the event that a Chinese paddlefish is unexpectedly caught in the near future, we aimed to develop an experimental strategy to produce paddlefish gametes in the gonads of surrogate sturgeon.

Late embryogenesis abundant group3 protein (DrLEA3) is involved in antioxidation in the extremophilic bacterium Deinococcus radiodurans.

Deinococcus radiodurans is able to survive under extreme conditions, including high doses of ionizing radiation, desiccation and oxidative stress. In addition to enhanced DNA repair capabilities, an effective antioxidation system plays an important role in its robustness. Previous studies have linked the radiation resistance of D.

Succinylome Analysis Reveals the Involvement of Lysine Succinylation in the Extreme Resistance of Deinococcus radiodurans.

Increasing evidence shows that the succinylation of lysine residues mainly regulates enzymes involved in the carbon metabolism pathway, in both prokaryotic and eukaryotic cells. Deinococcus radiodurans is one of the most radioresistant organisms on earth and is famous for its robust resistance. A major goal in the current study of protein succinylation is to explore its function in D.

Molybdenum Disulfide Quantum Dots Prepared by Bipolar-Electrode Electrochemical Scissoring.

A convenient bipolar-electrode (BPE) electrochemical method was engineered to produce molybdenum disulfide (MoS) quantum dots (QDs) using pure phosphate buffer (PBS) as the electrolyte and the MoS powder as the precursor. Meanwhile, the corresponding by-product precipitate was studied, in which MoS nanosheets were observed. The BPE design would not be restricted by the shape and size of the MoS precursor.

Facile Preparation of Snowflake-Like MnO @NiCo O Composites for Highly Efficient Electromagnetic Wave Absorption.

Snowflake-like MnO @NiCo O composites were successfully fabricated by employing crossed snowflake-like MnO nanorods as cores and one-dimensional (1D) NiCo O nanoneedles as shells. Impressively, the MnO @NiCo O composites exhibited a highly efficient electromagnetic wave (EMW) absorbing capability, and the minimum reflection loss (RL) value reached -58.4 dB at 6.

Ultra-efficient electromagnetic wave absorption with ethanol-thermally treated two-dimensional NbCT nanosheets.

NbCT, an emerging type of MXene, should be a promising electromagnetic wave (EMW) absorbing material to overcome the EMW pollution nowadays due to its unique layered structure and extremely thin monolayer thickness, but was lack of systematic study till now. Meanwhile, NbCT nanosheets obtained upon HF etching of NbAlC MAX was unfortunately found with limited absorption performance due to its mainly dielectric loss mechanism herein. Therefore, the NbCT nanosheets were further treated with solvothermal strategy in various solvents.

Characterization and role of a 2',3'-cyclic phosphodiesterase from Deinococcus radiodurans.

Objectives: A 2',3'-cyclic phosphodiesterase gene (drCPDase) has been characterized from Deinococcus radiodurans and is involved in the robust resistance of this organism.

Results: Cells lacking 2',3'-cyclic phosphodiesterase gene (drCPDase) showed modest growth defects and displayed increased sensitivities to high doses of various DNA-damaging agents including ionizing radiation, mitomycin C, UV and HO. The transcriptional level of drCPDase increased after HO treatment.

Crystal structure of the RNA 2',3'-cyclic phosphodiesterase from Deinococcus radiodurans.

2',3'-Cyclic phosphodiesterase (CPDase) homologues have been found in all domains of life and are involved in diverse RNA and nucleotide metabolisms. The CPDase from Deinococcus radiodurans was crystallized and the crystals diffracted to 1.6 Å resolution, which is the highest resolution currently known for a CPDase structure.

Structural insights into catalysis and dimerization enhanced exonuclease activity of RNase J.

RNase J is a conserved ribonuclease that belongs to the β-CASP family of nucleases. It possesses both endo- and exo-ribonuclease activities, which play a key role in pre-rRNA maturation and mRNA decay. Here we report high-resolution crystal structures of Deinococcus radiodurans RNase J complexed with RNA or uridine 5'-monophosphate in the presence of manganese ions.