Establishment and application of a rapid visual detection method for Clostridium perfringens in chicken products based on helical loop-mediated isothermal amplification (HAMP).

Clostridium perfringens is a significant foodborne pathogen in chicken products. Rapid on-site detection of C. perfringens is crucial for mitigating the incidence of foodborne illnesses by enabling the prompt identification and recall of contaminated food products.

Overexpression of the Kiwifruit Transcription Factor Decreases the Cold Tolerance in .

Cold stress is one of the main abiotic stresses that affect the development and growth of kiwifruit (). Herein, we analyzed the transcriptomic data of dormant shoots in response to low-temperature treatment, identified 52 MYB genes, and constructed a phylogenetic tree based on the encoded protein sequences. Then, the effect of one gene on cold tolerance was analyzed.

Synchronized autologous T-cell immunotherapy with hyperthermia to previously heavy treated advanced renal cell carcinoma.

Purpose: Immune checkpoint inhibitors (ICIs) and target therapy have provided the clinical efficacy for improving the clinical progression-free survival (PFS) and overall survival (OS) for patients with renal cell carcinoma (RCC). There has been little report of an surrogate salvage treatment for those failure of both ICIs and target. An innovation therapeutic model named SHAPE-T (synchronized hyperthermia with autologous progenitor expanded T cells) was applied to previously heavily treated metastatic RCC (mRCC).

Effect of low dose prednisone on glucose metabolism levels in patients with spontaneous abortion: A single-center, prospective cohort study.

In this single-center, prospective cohort study, we aimed to explore the effect of low dose prednisone treatment during pregnancy on blood glucose levels in patients with spontaneous abortion. Patients with a history of spontaneous abortion were enrolled and were assigned to two groups according to whether they were exposed to low dose prednisone during pregnancy. All patients received OGTT at early and late pregnancy.

Repurposing Benzbromarone as an Antibacterial Agent against Gram-Positive Bacteria.

The rise of antibiotic-resistant Gram-positive pathogens, particularly methicillin-resistant (MRSA), presents a significant challenge in clinical settings. There is a critical need for new antibacterial agents to combat these resistant strains. Our study reveals that the uricosuric drug Benzbromarone (Benz) exhibits potent antibacterial activity against Gram-positive pathogens, with minimum inhibitory concentrations (MICs) ranging from 8 to 32 μg/mL and minimum bactericidal concentrations (MBCs) ranging from 32 to 128 μg/mL against clinical isolates of , , , and .

Estimation and attribution of nonlinear trend of water use efficiency using a normalized partial derivative approach.

Investigating trends in water use efficiency (WUE) and its causality is critical for understanding ecosystem behaviors. Although WUE has shown nonlinear changes in the last several decades across most global ecosystems, the majority of available studies have focused on its linear trend. This study attempted to accurately attribute the linear and nonlinear variations in WUE using normalized driving factors in the Partial Derivative (PD) equation to develop a Normalized Partial Derivative model (NPD-model).

Live-Cell Imaging of miRNAs with the Combination of CHA and HCR Techniques.

The abnormal expression of miRNA-21 is closely related to many cancers, and its sensitive detection plays an important role in the diagnosis and treatment of cancers. In the report, we designed a non-enzymatic amplification sensing system based on the catalytic hairpin assembly (CHA) and palindrome-based hybridization chain reaction (PHCR) technique for the detection and imaging of miRNA in living cells. Based on PHCR technology, two ingeniously designed palindrome-contained fluorescently labeled hairpin probes are sequentially opened upon the stimulation of short oligonucleotide triggers, promoting the autonomous assembly of cross-linked network-like structures (CNSs) and generating fluorescence resonance energy transfer (FRET) signal.

Enhancing computational efficiency in topology-optimized mode converters via dynamic update rate strategies.

In the big data era, mode division multiplexing, as a technology for extended channel capacity, demonstrates potential in enhancing parallel data processing capability. Consequently, developing a compact, high-performance mode converter through efficient design methods is an urgent requirement. However, traditional design methodologies for these converters face significant computational complexities and inefficiencies.

Carbon-anchoring synthesis of PtNi@Pt/C core-shell catalysts for stable oxygen reduction reaction.

Proton-exchange-membrane fuel cells demand highly efficient catalysts for the oxygen reduction reaction, and core-shell structures are known for maximizing precious metal utilization. Here, we reported a controllable "carbon defect anchoring" strategy to prepare PtNi@Pt/C core-shell nanoparticles with an average size of ~2.6 nm on an in-situ transformed defective carbon support.

Vitamin U alleviates AFB-induced hepatotoxicity in pregnant and lactating mice by regulating the Nrf2/Hmox1 pathway.

This study investigated the protective effect of Vitamin U on liver injury induced by aflatoxin B (AFB) in maternal mice. 25 pregnant ICR mice were randomly divided into five groups: the AFB group (AF, 0.3 mg AFB/kg b.

Symmetry Donor-Acceptor Molecule Regulating Hydrogen-Bonding Network for Improved Metal Zinc Anode.

The issues of zinc dendrites and side reactions caused by active water molecules have seriously affected the development of aqueous zinc batteries (AZBs). Herein, a symmetry hydrogen-bond donor-acceptor molecule additive named 1,3-bis(hydroxymethyl)urea (BHMU) can preferentially adsorb on the anode surface and lock up water molecules through hydrogen bonding, thus isolating water molecules and reducing side reactions caused by active water molecules. With these advantages, the mixed electrolyte containing BHMU additive impels a reversible Zn anode with a high Coulombic efficiency (99.

Decoding the Temporal Structures and Interactions of Multiple Face Dimensions Using Optically Pumped Magnetometer Magnetoencephalography (OPM-MEG).

Humans possess a remarkable ability to rapidly access diverse information from others' faces with just a brief glance, which is crucial for intricate social interactions. While previous studies using event-related potentials/fields have explored various face dimensions during this process, the interplay between these dimensions remains unclear. Here, by applying multivariate decoding analysis to neural signals recorded with optically pumped magnetometer magnetoencephalography, we systematically investigated the temporal interactions between invariant and variable aspects of face stimuli, including race, gender, age, and expression.

Development and collaborative validation of an event-specific quantitative real-time PCR method for detection of genetically modified CC-2 maize.

As one of the developed genetically modified (GM) maize varieties in China, CC-2 has demonstrated promising commercial prospects during demonstration planting. The establishment of detection methods is a technical prerequisite for effective supervision and regulation of CC-2 maize. In this study, we have developed an event-specific quantification method that targets the junction region between the exogenous gene and the 5' flanking genomic DNA (gDNA) of CC-2.

A Lightweight Pathological Gait Recognition Approach Based on a New Gait Template in Side-View and Improved Attention Mechanism.

As people age, abnormal gait recognition becomes a critical problem in the field of healthcare. Currently, some algorithms can classify gaits with different pathologies, but they cannot guarantee high accuracy while keeping the model lightweight. To address these issues, this paper proposes a lightweight network (NSVGT-ICBAM-FACN) based on the new side-view gait template (NSVGT), improved convolutional block attention module (ICBAM), and transfer learning that fuses convolutional features containing high-level information and attention features containing semantic information of interest to achieve robust pathological gait recognition.

Biocatalytic Desymmetrization for the Atroposelective Synthesis of Axially Chiral Biaryls Using an Engineered Imine Reductase.

The enzymatic atroposelective synthesis of biaryl compounds is relatively rare, despite considerable attention received by biocatalysis in the academic and industrial sectors. Imine reductases (IREDs) are an important class of enzymes that have been applied in the asymmetric synthesis of chiral amine building blocks. In this study, two IREDs (IR140 and IR189) were identified to catalyze the efficient desymmetrization of biaryls utilizing various amine donors.

Stereoselective Synthesis of Oxetanes Catalyzed by an Engineered Halohydrin Dehalogenase.

Although biocatalysis has garnered widespread attention in both industrial and academic realms, the enzymatic synthesis of chiral oxetanes remains an underdeveloped field. Halohydrin dehalogenases (HHDHs) are industrially relevant enzymes that have been engineered to accomplish the reversible transformation of epoxides. In this study, a biocatalytic platform was constructed for the stereoselective kinetic resolution of chiral oxetanes and formation of 1,3-disubstituted alcohols.

Synergistic Sr Activation and Cr Buffering Effect on RuO Electronic Structures for Enhancing the Acidic Oxygen Evolution Reaction.

The oxygen evolution reaction (OER) performance of ruthenium-based oxides strongly correlates with the electronic structures of Ru. However, the widely adopted monometal doping method unidirectionally regulates only the electronic structures, often failing to balance the activity and stability. Here, we propose an "elastic electron transfer" strategy to achieve bidirectional optimization of the electronic structures of Sr, Cr codoped RuO catalysts for acidic OER.

Triangle-toothed gear occlude-guided universal nanotechnology constructs 3D symmetric DNA polyhedra with high assembly efficiency for precision cancer therapy.

Chemotherapy is commonly used to treat malignant tumors. However, conventional chemotherapeutic drugs often cannot distinguish between tumor and healthy cells, resulting in adverse effects and reduced therapeutic efficacy. Therefore, zigzag-shaped gear-occlude-guided cymbal-closing (ZGC) DNA nanotechnology was developed based on the mirror-symmetry principle to efficiently construct symmetric DNA polyhedra.

Research into the Applications of a Multi-Scale Feature Fusion Model in the Recognition of Abnormal Human Behavior.

Due to the increasing severity of aging populations in modern society, the accurate and timely identification of, and responses to, sudden abnormal behaviors of the elderly have become an urgent and important issue. In the current research on computer vision-based abnormal behavior recognition, most algorithms have shown poor generalization and recognition abilities in practical applications, as well as issues with recognizing single actions. To address these problems, an MSCS-DenseNet-LSTM model based on a multi-scale attention mechanism is proposed.

Rationally Engineered Novel Glycosyltransferase UGT74DD1 from Catalyzes the Generation of the Sweetener Mogroside III.

Mogrosides are natural compounds highly valued in the food sector for their exceptional sweetness. Here, we report a novel -glycosyltransferase (UGT74DD1) from that catalyzes the conversion of mogrol to mogroside IIE. Site-directed mutagenesis yielded the UGT74DD1-W351A mutant, which exhibited the new capability to transform mogroside IIE into the valuable sweetener mogroside III, but with low catalytic activity.