Development and application of an indirect ELISA to detect antibodies to in cattle based on a chimeric protein rSRS2-SAG1-GRA7.

is an important apicomplexan parasite causing neosporosis in cattle. The disease is recognized as one of the most important cause of reproductive problems and abortion in cattle worldwide. In this context, we developed an indirect enzyme-linked immunosorbent assays (ELISA) with chimeric protein rSRS2-SAG1-GRA7 to diagnose antibodies to -infection.

mTORC2 Activation Mediated by Mesenchymal Stem Cell-Secreted Hepatocyte Growth Factors for the Recovery of Lipopolysaccharide-Induced Vascular Endothelial Barrier.

Acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) is characterized by pulmonary microvascular endothelial barrier dysfunction. Mesenchymal stem cell-secreted hepatocyte growth factor (HGF) has positive effects of lipopolysaccharide- (LPS-) induced pulmonary endothelial barrier. Studies have exhibited the mammalian TORC1 (mTORC1) signaling is of potent angiogenesis effects.

Nucleotide polymorphism in ARDS outcome: a whole exome sequencing association study.

Background: Genetic locus were identified associated with acute respiratory distress syndrome (ARDS). Our goal was to explore the associations between genetic variants and ARDS outcome, as well as subphenotypes.

Methods: This was a single-center, prospective observational trial enrolling adult ARDS patients.

Effects of Propofol on Respiratory Drive and Patient-ventilator Synchrony during Pressure Support Ventilation in Postoperative Patients: A Prospective Study.

Background: Propofol is increasingly used during partial support mechanical ventilation such as pressure support ventilation (PSV) in postoperative patients. However, breathing pattern, respiratory drive, and patient-ventilator synchrony are affected by the sedative used and the sedation depth. The present study aimed to evaluate the physiologic effects of varying depths of propofol sedation on respiratory drive and patient-ventilator synchrony during PSV in postoperative patients.

Hepatic Perfusion Alterations in Septic Shock Patients: Impact of Early Goal-directed Therapy.

Background: Early goal-directed therapy (EGDT) has become an important therapeutic management in early salvage stage of septic shock. However, splenic organs possibly remained hypoperfused and hypoxic despite fluid resuscitation. This study aimed to evaluate the effect of EGDT on hepatic perfusion in septic shock patients.

Comparison of the effects of albumin and crystalloid on mortality in adult patients with severe sepsis and septic shock: a meta-analysis of randomized clinical trials.

Introduction: The aim of this study was to examine whether albumin reduced mortality when employed for the resuscitation of adult patients with severe sepsis and septic shock compared with crystalloid by meta-analysis.

Methods: We searched for and gathered data from MEDLINE, Elsevier, Cochrane Central Register of Controlled Trials and Web of Science databases. Studies were eligible if they compared the effects of albumin versus crystalloid therapy on mortality in adult patients with severe sepsis and septic shock.

[Assessment of central venous-to-arterial CO(2) difference during early goal-directed therapy in patients with septic shock].

Objective: To detect the changes of central venous-to-arterial carbon dioxide difference (P(cv-a)CO(2)) during early goal-directed therapy (EGDT) in patients with septic shock and evaluate its' value in predicting adequate resuscitation and prognosis.

Methods: From April 2009 to October 2010, 26 septic shock patients were enrolled in the study. EGDT was performed in all the patients immediately after enrollment.

[The effect of stress dose glucocorticoid on patients with acute respiratory distress syndrome combined with critical illness-related corticosteroid insufficiency].

Objective: To investigate the effect of stress dose glucocorticoid on patients with acute respiratory distress syndrome (ARDS) combined with critical illness-related corticosteroid insufficiency (CIRCI).

Methods: All early ARDS patients combined with CIRCI were screened by an adrenal corticotrophic hormone (ACTH) test and randomly divided into treatment group (hydrocortisone 100 mg intravenous, 3 times/day, consecutively for 7 days, n = 12) and control group (equivalent normal saline, n = 14). General clinical data, changes of arterial blood gas, hemodynamics and respiratory mechanics were observed and recorded at admission and at 7 days after treatment.

[The evaluation value of severity and prognosis of septic shock patients based on the arterial-to-venous carbon dioxide difference].

Objective: To assess the value of central venous-to-arterial carbon dioxide difference [P((cv-a))CO₂] in evaluation of disease severity and prognosis in patients with septic shock.

Methods: There were 45 consecutive resuscitated septic shock patients from April 2009 to October 2010 included immediately after their admission into our ICU. The patients were divided into low P((cv-a))CO₂ group and high P((cv-a))CO₂ group according to a threshold of 6 mm Hg (1 mm Hg = 0.

[The clinical application of pulmonary vascular permeability index on differential diagnosis of acute pulmonary edema].

Objective: To assess the value of pulmonary vascular permeability index in differentiating acute lung injury (ALI) from cardiac pulmonary edema.

Methods: Critically ill patients with acute pulmonary edema were included from May, 2004 to September, 2008. Patients were divided into two groups, the ALI group and the cardiac pulmonary edema group (C group).

[Effect of conservative fluid management strategy on the outcomes in patients with acute lung injury: a meta-analysis].

Objective: To evaluate the effect of conservative fluid management on the outcomes in patients with acute lung injury (ALI).

Methods: Randomized controlled trials (RCTs) and cohort trials on conservative fluid management from January 1, 1990 to May 20, 2010 were retrieved by electronic and manual searching. A meta-analysis of effect of conservative fluid management on the outcomes in ALI patients was conducted by the methods as recommended by the Cochrane Collaboration.

[Effects of different reference point on intra-abdominal pressure measurement in critically ill patients: a clinical trial].

Objective: To investigate the effects of different reference point on intra-abdominal pressure (IAP) measurement.

Methods: Nine critically ill patients with risk of intra-abdominal hypertension (IAH) were studied from July 2008 to June 2010, all of the patients were equipped with abdominal cavity drain and urinary drainage tube. By which IAP was measured using direct and indirect methods respectively.

[Passive leg raising predicts volume responsiveness in patients with septic shock].

Objective: To evaluate the hemodynamic response to passive leg raising (PLR) indicates fluid responsiveness in patients with septic shock.

Methods: Twenty patients with septic shock, considered for fluid challenge (FC), were enrolled in the study from June 2009 to May 2010. Hemodynamic changes were determined by pulse-contour derived cardiac index at baseline, before and after PLR, return to baseline for 10 min, before and after fluid challenge (250 ml saline for 10 min).

[Effect of lung stress index upon titration of positive end-expiratory pressure at post-recruitment in patients with acute respiratory distress syndrome].

Objective: To investigate the relationship of stress index and positive end-expiratory pressure (PEEP) in patients with acute respiratory distress syndrome (ARDS) and to determine the relationship of stress index, lung recruitment, oxygenation and respiration mechanics.

Methods: Fourteen ARDS patients were enrolled. During volume control ventilation with constant inspiratory flow, the pressure-time (P-t) curve was fitted to a power equation: P = a x time(b) + c, where coefficient b (stress index) describes the shape of curve: b = 1, straight curve; b < 1, progressive increase in slope; and b > 1, progressive decrease in slope.

[Prospective research on the prognosis of septic shock based on the change of lactate concentration in arterial blood].

Objective: To evaluate the correlation between the lactate in artery blood and lactate clearance rate and prognosis in patients with septic shock in intensive care unit (ICU).

Methods: Prospectively studied 221 consecutive patients with septic shock from December 2005 to December 2007, the diagnosis of septic shock was made based on the criteria of 2001 ACCP/SCCM. For inclusion in the study, we required admission of the patients within 24 h of septic shock diagnosed.

[Effect of continuous aspiration of subglottic secretions on the prevention of ventilator-associated pneumonia in mechanically ventilated patients: a prospective, randomized, controlled clinical trial].

Objective: To evaluate the effect of continuous aspiration of subglottic secretions (CASS) on the prevention of ventilator-associated pneumonia (VAP) in mechanically ventilated patients.

Methods: Patients ventilated mechanically at the ICU from October, 2004 to April, 2006 were randomly divided into 2 groups: one group received CASS and the other did not (NASS group). CASS was performed immediately after admission for patients in the CASS group.

[The prognostic value of extravascular lung water index in critically ill septic shock patients].

Objective: To investigate the prognostic value of extravascular lung water index (EVLWI) in critically ill patients with septic shock in intensive care unit (ICU).

Methods: EVLWI was determined by using a PiCCO Monitor, and the daily fluid balance was recorded.

Results: Fifty patients with septic shock were admitted and twenty-six patients survived.