Phytohalomonas tamaricis gen. nov., sp. nov., an endophytic bacterium isolated from Tamarix ramosissima roots growing in Kumtag desert.

A gram-stain-negative, aerobic, non-spore-forming, rod-shaped, non-motile bacterium strain R4HLG17 was isolated from Tamarix ramosissima roots growing in Kumtag desert. The strain grew at salinities of 0-16% (w/v) NaCl (optimum 5-6%), pH 5-9 (optimum 7) and at 16-45 °C. Based on 16S rRNA gene sequence similarity, strain R4HLG17 belonged to the family Halomonadaceae and was most closely related to Halomonas lutea DSM 23508(95.

The protein elicitor Hrip1 enhances resistance to insects and early bolting and flowering in Arabidopsis thaliana.

The elicitor Hrip1 isolated from necrotrophic fungus Alternaria tenuissima, could induce systemic acquired resistance in tobacco to enhance resistance to tobacco mosaic virus. In the present study, we found that the transgenic lines of Hrip1-overexpression in wild type (WT) Arabidopsis thaliana were more resistant to Spodoptera exigua and were early bolting and flowering than the WT. A profiling of transcription assay using digital gene expression profiling was used for transgenic and WT Arabidopsis thaliana.

Application of lipidomics to reveal differences in facial skin surface lipids between males and females.

Purpose: To analyze and compare differences in facial skin surface lipids (SSL) between 18- to 25-year-old males and females.

Methods: Ultra-performance liquid chromatography quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS) technology was used to measure the facial SSL composition of 18- to 25-year-old males and females. Measurement results were combined with the orthogonal projections to latent structures discriminant analysis (OPLS-DA) model for analysis and comparisons, and differences in lipids with significance were selected.

Advancements in the maintenance of skin barrier/skin lipid composition and the involvement of metabolic enzymes.

The human skin barrier has an important role in protection and defense, reflected not only in the ability to resist entry of harmful substances into the human body, but also in the ability to prevent loss of water and nutrients. Once the skin barrier is damaged, the skin may become dry, scaly, and wrinkled, and a series of skin problems may occur. In this article, we review the composition of lipids, such as ceramides, cholesterol, and free fatty acids, in the skin and examine the expression of enzymes related to lipid metabolism, such as kallikreins, elongase of elongation of very long-chain fatty acids, hydrolases, and lipid synthases.

[Preliminary Studies on the Relationship Between Meiosis of Pollen Mother Cells and Pollen Abortion of Aloe arboresense Mill.].

The main reason for pollen abortion in Aloe arboresens Mill. was studied through the observation of meiosis and the microspore development of its pollen mother cells(PMCs). There are 14 chromosomes in the PMC of Aloe arboresens Mill.

PCR-based screening BAC library and direct end sequencing of BAC clones.

A PCR based strategy was developed which required four steps to identify positive BAC clones from barley BAC (bacterial artificial chromosome) library. In the protocol, two levels of BAC DNA pools (super-pool and pool) were prepared for analysis. One pool is made of one plate DNAs and one super-pool is made of mixing ten consecutive 1/100 diluted pool DNAs (1 approximately 10, 11 approximately 20 ect).