Integrated colon microbiome and metabolomics to elucidate the antidepressant mechanisms of the Radix Bupleuri-Radix Paeoniae Alba herb pair.

Radix Bupleuri-Radix Paeoniae Alba herb pair (RB-RPA) is the fundamental medication combination of many classic antidepressant prescriptions, and RB-RPA's antidepressant effect is well established. For an extended period, the involvement of intestinal flora in the progression of depression has been widely acknowledged. However, it remains unclear whether RB-RPA could modulate intestinal microbiota disturbances and metabolic abnormalities induced by depression.

PADI1 and Its Co-Expressed Gene Signature Unveil Colorectal Cancer Prognosis and Immunotherapy Efficacy.

Peptidyl arginine deiminase 1 (PADI1) catalyzes protein citrullination and has a role in regulating immune responses. The tumor immune microenvironment has been reported to be important in colorectal cancer (CRC), which was correlated with the ability of CRC patients to benefit from immunotherapy. However, there is a lack of molecular markers for matching CRC immunotherapy.

Three Silver(I) Coordination Polymers Based on Pyridyl Ligands and Auxiliary Carboxylic Ligands: Luminescence and Efficient Sensing Properties.

Easily producible sensors for harmful industrial waste compounds are of significant interest for both human health and the environment. Three novel coordination polymers, [Ag(μ-aca)(μ-bztpy)] (), [Ag(μ-bza)(μ-bpa)] (), and [Ag(μ-aca)(μ-bpa)]·EtOH·2HO (), were assembled in this study by reactions using Ag as a node with the pyridyl ligand 1,2,4,5-tetrakis(4-pyridyl)benzene (bztpy) or 9,10-bis(4-pyridyl)anthracene (bpa) and an auxiliary chelating carboxylic ligand. Single-crystal X-ray structural analyses revealed that compound has a 3D framework consisting of 1D [Ag(aca)] chains and bztpy linkers, while and have 2D layered structures consisting of binuclear Ag-carboxylate units and bpa linkers, respectively.

Facile Chemoenzymatic Synthesis of O-Mannosyl Glycans.

O Mannosylation is a vital protein modification involved in brain and muscle development whereas the biological relevance of O-mannosyl glycans has remained largely unknown owing to the lack of structurally defined glycoforms. An efficient scaffold synthesis/enzymatic extension (SSEE) strategy was developed to prepare such structures by combining gram-scale convergent chemical syntheses of three scaffolds and strictly controlled sequential enzymatic extension catalyzed by glycosyltransferases. In total, 45 O-mannosyl glycans were obtained, covering the majority of identified mammalian structures.

[Seasonal Variation on Nutrient Limitation for Phytoplankton Growth in a Coastal River-Reservoir System, Southeast China].

A comprehensive analysis was conducted using a dataset obtained from October in 2013 to October in 2014 monitoring in 20 headwater streams of Jiulong River and four reservoirs, situated in such a coastal river-reservoir system in Southeast China suffering from intensive anthropogenic disturbance. In-situ monitoring, GIS and statistical analysis were coupled in this study to identify the spatiotemporal variations of nutrients & phytoplankton abundance and community structure, the differentiation of nitrogen & phosphorus limitation of phytoplankton growth, and the seasonal variations in nutrient limitation of phytoplankton growth. The results showed that there were obvious spatiotemporal variations in terms of nutrients & phytoplankton abundance and community structure in the 20 headwater streams and four reservoirs.

Postconditioning of sevoflurane and propofol is associated with mitochondrial permeability transition pore.

Background: Sevoflurane and propofol are effective cardioprotective anaesthetic agents, though the cardioprotection of propofol has not been shown in humans. Their roles and underlying mechanisms in anesthetic postconditioning are unclear. Mitochondrial permeability transition pore (MPTP) opening is a major cause of ischemia-reperfusion injury.

Propofol attenuation of renal ischemia/reperfusion injury involves heme oxygenase-1.

Aim: To examine the protective effect of propofol in renal ischemia/reperfusion (I/R) injury and the role of heme oxygenase-1 (HO-1) in this process.

Methods: Sprague-Dawley rats were randomly divided into 3 groups: (i) sham-operated group; (ii) I/R group; and (iii) propofol group. Bilateral renal warm ischemia for 45 min was performed.

Effects of ulinastatin on renal ischemia-reperfusion injury in rats.

Aim: To investigate the effect and possible mechanism of ulinastatin on renal ischemia-reperfusion injury in rats.

Methods: Male Sprague-Dawley rats were subjected to 45-min bilateral renal ischemia, treated with intravenously 12,500 U ulinastatin at 30 min prior to ischemia and at the beginning of reperfusion, compared with a nontreated group without ulinastatin and a sham-operation group without bilateral renal ischemia. After 0 h, 2 h, 6 h, 12 h, and 24 h of reperfusion, serum creatinine and blood urea nitrogen were measured for the assessment of renal function, renal sections were used for histologic grading of renal injury, for immunohistochemical localization of Bcl-2 and heat shock protein 70.

[Effects of ulinastatin on renal ultrastructure after ischemia-reperfusion in rats].

Objective: To investigate the effect of ulinastatin on renal function and ultrastructure changes after renal ischemia-reperfusion in rats.

Methods: Acute ischemic renal injury model was established (45 min of bilateral renal ischemia and reperfusion for 24 h). Thirty Male SD rats were randomly divided into 3 groups: sham operation group (control group or group C, without renal ischemia), renal ischemia-reperfusion group (ischemia-reperfusion group or group I, without ulinastatin), renal ischemia-reperfusion and ulinastatin intravenous injection group (ulinastatin group or group U).