Microsporidian EnP1 alters host cell H2B monoubiquitination and prevents ferroptosis facilitating microsporidia survival.

Microsporidia are intracellular eukaryotic pathogens that pose a substantial threat to immunocompromised hosts. The way these pathogens manipulate host cells during infection remains poorly understood. Using a proximity biotinylation strategy we established that microsporidian EnP1 is a nucleus-targeted effector that modifies the host cell environment.

Autonomous Exploration Method of Unmanned Ground Vehicles Based on an Incremental B-Spline Probability Roadmap.

Autonomous exploration in unknown environments is a fundamental problem for the practical application of unmanned ground vehicles (UGVs). However, existing exploration methods face difficulties when directly applied to UGVs due to limited sensory coverage, conservative exploration strategies, inappropriate decision frequencies, and the non-holonomic constraints of wheeled vehicles. In this paper, we present IB-PRM, a hierarchical planning method that combines Incremental B-splines with a probabilistic roadmap, which can support rapid exploration by a UGV in complex unknown environments.

RLAD: A Reliable Hippo-guided Multi-task Model for Alzheimer's Disease Diagnosis.

Early diagnosis of Alzheimer's disease (AD) is crucial for its prevention, and hippocampal atrophy is a significant lesion for early diagnosis. The current DL-based AD diagnosis methods only focus on either AD classification or hippocampus segmentation independently, neglecting the correlation between the two tasks and lacking pathological interpretability. To address this issue, we propose a Reliable Hippo-guided Learning model for Alzheimer's Disease diagnosis (RLAD), which employs multi-task learning for AD classification as a main task supplemented by hippocampus segmentation.

LettuceDB: an integrated multi-omics database for cultivated lettuce.

Crop genomics has advanced rapidly during the past decade, which generated a great abundance of omics data from multi-omics studies. How to utilize the accumulating data becomes a critical and urgent demand in crop science. As an attempt to integrate multi-omics data, we developed a database, LettuceDB (https://db.

[Analysis of O Pollution Affected by a Succession of Three Landfall Typhoons in 2020 in Eastern China].

Based on air quality monitoring, surface meteorological data, wind profile radar observation, and the HYSPLIT model, the characteristics and causes of O pollution in eastern China during the period of the typhoons BAVI, MAYSAK, and HAISHEN from August 26 to September 8, 2020 were analyzed. The results showed that during the succession of the three landfall typhoons, the O pollution sites in Beijing Tianjin Hebei and its surrounding areas (BTHS) and the Yangtze River Delta (YRD) exceeded 50%. During the HAISHEN period, O pollution days in the two regions reached 2.

From O/GABA-AT, GABA, and T-263 Mutant to Conception of .

causes morbidity, mortality, and disseminates widely via cat sexual stages. Here, we find ornithine aminotransferase (OAT) is conserved across phyla. We solve O/GABA-AT structures with bound inactivators at 1.

Transcriptome Identification and Analysis of Fatty Acid Desaturase Gene Expression at Different Temperatures in 6A7.

6A7 is a low-temperature yeast strain that can produce lipases. Yeast, which is made up of chassis cells, is an important part of synthetic biology, and the use of the lipase-producing properties of 6A7 for the production of fatty acids provides a new pathway for targeted synthesis in yeast cell factories. In this study, we performed RNA-seq on lipase-producing 6A7 at different temperatures (15 °C, 20 °C, 20 °C without corn oil, and 25 °C).

Melatonin alleviates particulate matter-induced liver fibrosis by inhibiting ROS-mediated mitophagy and inflammation via Nrf2 activation.

Objective: Fine particulate matter (PM2.5) is a source of pollution worldwide, that causes inflammation and liver fibrosis. Melatonin, as the predominant hormone secreted by the pineal gland, can inhibit PM2.

Hierarchical Set-to-Set Representation for 3-D Cross-Modal Retrieval.

Three-dimensional in-domain retrieval has recently achieved significant success, but 3-D cross-modal retrieval still faces problems and challenges. Existing methods only rely on a simple global feature (GF), which overlooks the local information of complex 3-D objects and the connections between similar local features across complex multimodal instances. To tackle this issue, we propose a hierarchical set-to-set representation (HSR) and a corresponding hierarchical similarity that incorporates global-to-global and local-to-local similarity metrics.

STOmicsDB: a comprehensive database for spatial transcriptomics data sharing, analysis and visualization.

Recent technological developments in spatial transcriptomics allow researchers to measure gene expression of cells and their spatial locations at the single-cell level, generating detailed biological insight into biological processes. A comprehensive database could facilitate the sharing of spatial transcriptomic data and streamline the data acquisition process for researchers. Here, we present the Spatial TranscriptOmics DataBase (STOmicsDB), a database that serves as a one-stop hub for spatial transcriptomics.

Gaze Point Tracking Based on a Robotic Body-Head-Eye Coordination Method.

When the magnitude of a gaze is too large, human beings change the orientation of their head or body to assist their eyes in tracking targets because saccade alone is insufficient to keep a target at the center region of the retina. To make a robot gaze at targets rapidly and stably (as a human does), it is necessary to design a body-head-eye coordinated motion control strategy. A robot system equipped with eyes and a head is designed in this paper.

Daphnetin ameliorates PM2.5-induced airway inflammation by inhibiting NLRP3 inflammasome-mediated pyroptosis in CS-exposed mice.

Epidemiologic studies have shown that fine particulate matter 2.5 (PM2.5) exaggerates airway inflammation associated with acute exacerbation of chronic obstructive pulmonary disease (AECOPD).

Influence of in the Regulation of Antioxidant Function Melanin Biosynthesis in .

We have successfully identified the transcription factor from the fungus Hit-lcy3T, which regulates melanin biosynthesis genes. Bioinformatics analysis revealed that the Cmr1 gene encodes a protein of 945 amino acids, containing two CysHis zinc finger domains and a Zn(II)Cys binuclear cluster domain located at the N-terminus of . To investigate the function of the gene, we performed gene knockout and overexpression experiments.

The role of pH transcription factor Appacc in upregulation of pullulan biosynthesis in Aureobasidium pullulans using potato waste as a substrate.

pH is one of the important environmental factors affecting the growth, development and secondary metabolites of fungi. To better utilize potato waste for the production of pullulan by fermentation, in this study, the amino acid sequence and structural domain of pH transcription factor Appacc were analyzed using the bioinformatics methods. Appacc showed three typically conserved zinc finger domains, with the closest homology to Zymoseptoria brevis.

Gene Regulates the Synthesis of β-Glucan with Antioxidant Activity in the .

The ability of the fungus to regulate metabolism on various nitrogen sources makes it survive and metabolize in different environments. The biomass and the β-glucan yield of are closely associated with the nitrogen source. This study found the only GATA nitrogen source activation regulating factor in HIT-LCY.

A redox switch regulates the assembly and anti-CRISPR activity of AcrIIC1.

Anti-CRISPRs (Acrs) are natural inhibitors of bacteria's CRISPR-Cas systems, and have been developed as a safeguard to reduce the off-target effects of CRISPR gene-editing technology. Acrs can directly bind to CRISPR-Cas complexes and inhibit their activities. However, whether this process is under regulation in diverse eukaryotic cellular environments is poorly understood.

Primary Malignant Brain Tumors following Systemic Malignancies: A Population-Based Analysis.

Background: Several reports have described glioma following different cancers. We assessed the prevalence of primary malignant brain tumors afterward systemic malignancies in patients in the USA based on Surveillance, Epidemiology, and End Results (SEER) program data.

Methods: The detailed data of patients with primary malignant brain tumors following an initial malignant tumor outside the central nervous system were extracted from SEER.

and anti- activities of HDAC inhibitor Panobinostat on experimental acute ocular toxoplasmosis.

Ocular toxoplasmosis (OT) is retinochoroiditis caused by infection, which poses a huge threat to vision. However, most traditional oral drugs for this disease have multiple side effects and have difficulty crossing the blood-retinal barrier, so the new alternative strategy is required to be developed urgently. Histone deacetylases (HDAC) inhibitors, initially applied to cancer, have attracted considerable attention as potential anti- drugs.

Screening and identification of a strain of Aureobasidium pullulans and its application in potato starch industrial waste.

Pullulan and melanin have become important secondary metabolites that are now widely studied. In this study, a strain of Aureobasidium pullulans HIT-LCY3 was used to ferment potato starch industrial waste to produce pullulan and melanin. After optimization, the culture conditions for the fermentation medium were obtained: inoculum age of 48 h, initial pH of 6.

Enhanced Anticancer Efficacy of Chemotherapy by Amphiphilic Y-Shaped Polypeptide Micelles.

Although the treatment modalities of cancers are developing rapidly, chemotherapy is still the primary treatment strategy for most solid cancers. The progress in nanotechnology provides an opportunity to upregulate the tumor suppression efficacy and decreases the systemic toxicities. As a promising nanoplatform, the polymer micelles are fascinating nanocarriers for the encapsulation and delivery of chemotherapeutic agents.

BNIP3 contributes to silibinin-induced DNA double strand breaks in glioma cells via inhibition of mTOR.

BNIP3 is found to eliminate cancer cells via causing mitochondrial damage and endoplasmic reticulum stress, but it remains elusive of its role in regulating DNA double strand breaks (DSBs). In this study, we find that silibinin triggers DNA DSBs, ROS accumulation and expressional upregulation of BNIP3 in glioma cells. Mitigation of ROS with antioxidant GSH significantly inhibits silibinin-induced DNA DSBs and glioma cell death.

Quantitative Peptidomics of Mouse Brain After Infection With Cyst-Forming .

is an obligate intracellular parasite capable of establishing persistent infection within the host brain and inducing severe neuropathology. Peptides are important native molecules responsible for a wide range of biological functions within the central nervous system. However, peptidome profiling in host brain during infection has never been investigated.

Upregulation of lncRNA147410.3 in the Brain of Mice With Chronic Infection Promoted Microglia Apoptosis by Regulating Hoxb3.

is neurotropic and affects the function of nerve cells, while the mechanism is unclear. LncRNAs are abundantly enriched in the brain and participated in the delicate regulation of the central nervous system (CNS) development. However, whether these lncRNAs are involved in the regulation of microglia activation during the process of infection is largely unknown.

Chromatin-regulating genes are associated with postoperative prognosis and isocitrate dehydrogenase mutation in astrocytoma.

Background: Abnormality in chromatin regulation is a major determinant in the progression of multiple neoplasms. Astrocytoma is a malignant histologic morphology of glioma that is commonly accompanied by chromatin dysregulation. However, the systemic interpretation of the expression characteristics of chromatin-regulating genes in astrocytoma is unclear.