Publications by authors named "Concetta Maria Messina"

Article Synopsis
  • The scientific community is increasingly focused on understanding the effects of various mixtures of pollutants and pharmaceuticals, which lack established evidence regarding their interactions and impacts on health.
  • This study aimed to explore how sub-lethal concentrations of certain emerging pollutants and drugs affect toxicity, oxidative stress, and cancer-related biomarkers using the HepG2 hepatoma cell line.
  • Results indicated that these mixtures can enhance the activation of pathways related to detoxification and oxidative stress, causing greater cytotoxic effects compared to exposure to individual compounds, highlighting their potential as early warning markers for environmental monitoring.
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The blue crab (), originally from the western Atlantic Ocean, has recently spread to the Mediterranean and is now considered one of the one hundred most invasive species in that region. This opportunistic species, known for its adaptability to different temperatures and salinities, negatively impacts biodiversity and human activities such as fishing and tourism in the Mediterranean. However, the blue crab is gaining interest as a potential food resource due to its high nutritional value and delicate, sweet flavor.

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  • * The effects of the probiotic inclusion were measured in terms of fish growth, immune responses (including humoral and cellular immunity), and bactericidal activity over 15 and 30 days.
  • * Results indicated that the higher probiotic inclusion (0.1%) improved growth performance and certain immune responses, suggesting the potential for better fish welfare and aquaculture practices through future dietary supplements.
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  • - The study investigates the impact of a mix of contaminants (carbamazepine, PBDE-47, and cadmium) on the fish species Sparus aurata over a 30-day period, focusing on both oxidative stress in the liver and changes in nutritional composition.
  • - After 15 days of exposure to the contaminants, key molecular markers indicating oxidative stress showed increased levels, while the fatty acid profile indicated a rise in saturated fats and a drop in polyunsaturated fats, alongside increased lipid damage (measured by MDA levels).
  • - The research implies that contaminants not only affect molecular health but also alter nutritional quality in fish, suggesting that monitoring these biochemical markers could be crucial for assessing the health of marine life
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L. is a dietary source that produces polyphenols as secondary metabolites. These natural compounds with potent antioxidant abilities are increasingly recommended as a supplement to inhibit oxidative stress.

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The valorization of side streams from fishery and aquaculture value-chains is a valuable solution to address one of the challenges of the circular economy: turning wastes into profit. Side streams produced after filleting of sea cage fattened bluefin tuna () were analyzed for proximate composition and fatty acid profile to evaluate the possibility of producing tuna oil (TO) as a valuable source of ω-3 polyunsaturated fatty acids (PUFA) and testing its bioactivity in vitro. Ethyl esters of total fatty acids (TFA), obtained from TO, were pre-enriched by urea complexation (PUFA-Ue) and then enriched by short path distillation (SPD) up to almost 85% of the PUFA fraction (PUFA-SPe).

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The awareness of the correlation between administered diet, fish health and products’ quality has led to the increase in the research for innovative and functional feed ingredients. Herein, a plant-derived product rich in bioactive compounds, such as honeybee pollen (HBP), was included as raw (HBP) and as Supercritical Fluid Extracted (SFE) pollen (HBP_SFE) in the diet for gilthead seabream (Sparus aurata). The experiment was carried out on 90 fish with an average body weight of 294.

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(Bacillariophyta) is a worldwide-distributed diatom with the ability to adapt and survive in different environmental habitats and nutrient-limited conditions. In this research, we investigated the growth performance, the total lipids productivity, the major categories of fatty acids, and the antioxidant content in subjected for 15 days to nitrogen deprivation (N-) compared to standard culture conditions (N+). Furthermore, genes and pathways related to lipid biosynthesis (i.

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Aquaculture has been playing a leading role over the years to satisfy the global growing demand for seafood. Moreover, innovative techniques are necessary to increase the competitiveness, sustainability and profitability of the seafood production chain, exploiting new species from the aquaculture, such as meagre (), to develop value-added products and diversify their production. In the present work, the effectiveness of cold smoking combined with antioxidants (SA) compared to cold smoking alone (S) on meagre fillets, the quality and shelf life were investigated.

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The marine environment is a generous source of biologically active compounds useful for human health. In 50 years, about 25,000 bioactive marine compounds have been identified, with an increase of 5% per year. Peculiar feature of algae and plants is the production of secondary metabolites, such as polyphenols, synthesized as a form of adaptation to environmental stress.

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Gill monogenean is considered the most detrimental fish parasite to the Mediterranean aquaculture. Treatment of sparicotylosis relies on frequent gill inspections correlated with the seasonal increase in seawater temperature, application of functional feeds, and treatments with formalin baths where permitted. While the latter is bound to be banned in Europe, other synthetic anthelminthics, such as praziquantel and ivermectin, are prone to induce resistance in the parasites.

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Non-edible parts of crustaceans could be a rich source of valuable bioactive compounds such as the carotenoid astaxanthin and peptides, which have well-recognized beneficial effects. These compounds are widely used in nutraceuticals and pharmaceuticals, and their market is rapidly growing, suggesting the need to find alternative sources. The aim of this work was to set up a pilot-scale protocol for the reutilization of by-products of processed shrimp, in order to address the utilization of this valuable biomass for nutraceutical and pharmaceuticals application, through the extraction of astaxanthin-enriched oil and antioxidant-rich protein hydrolysates.

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This study shows a pilot scale protocol aimed to obtain an omega 3-enriched oil after the processing of farmed gilthead sea bream viscera (SBV); this was oil was tested in vitro for bioactivity, attesting to the possibility to turn waste into profit The quality of the oil, in terms of requirements for animal and human consumption, was assessed by determining some chemical parameters, such as peroxide value (PV), thiobarbituric acid reactive substances (TBARS), ρ-anisidine (ρ-AV) content, total oxidation value (TOTOX), and phospholipids and free fatty acid (%), both in crude viscera oil (CVO) and refined viscera oil (RVO). Among the extraction conditions, the higher CVO yields were obtained at 60 °C for 10 min (57.89%) and at 80 °C for 10 min (67.

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Aquaculture is playing a leading role in both meeting the growing demand for seafood and increasing the sustainability of the fish production sector. Thus, innovative technologies that improve its sustainability, competitiveness, and safety are necessary for growth in the sector. This study aimed to develop cold smoked sea bass fillets from aquaculture.

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Article Synopsis
  • Polybrominated diphenyl ethers (PBDEs), particularly BDE-47, are stable flame retardants that negatively impact marine life, accumulating in organisms like mussels (Mytilus galloprovincialis) and causing toxicity and oxidative stress.
  • In the study, mussels were exposed to BDE-47 through microalgae for 15 days, followed by 15 days without the contaminant, during which gills and digestive glands were analyzed for biochemical changes and gene expression.
  • Results indicated that after 15 days of exposure, BDE-47 significantly disrupted cellular activity in mussels, particularly in the digestive gland, with long-lasting effects observed even after the contaminant was removed, raising concerns
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This study aimed to determine whether isotopic ratio mass spectrometry (IRMS) can discriminate farmed European sea bass according to different farming systems and geographic origins. of commercial size from three different rearing systems (concrete tank inland, sea cages, and extensive methods in valleys or salt works) were collected at the trading period (autumn-winter). For each farming type, different locations spread over Italy were monitored.

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The use of biological immunostimulants is considered a valuable practice to improve culture conditions in aquaculture sector that may help to increase production and maintain healthy environment. We undertook this study in order to evaluate the potential effect of the administration of two exopolysaccharides (EPS) "EPLB" and "EPB" derived from potential probiotic strains on immune and antioxidant status of European sea bass (Dicentrarchus labrax L.) larvae.

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In the present study, the immune-stimulatory effect of two levels of honey bee pollen (5 and 10%, P5 and P10 treatment, respectively) and its supercritical fluid extract (0. 5 and 1%, E0.5 and E1, respectively) included in the diet, was tested in gilthead seabream .

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Jasonia glutinosa (rock-tea, RT) has numerous biological activities. In the present work, the beneficial effects of dietary RT on gilthead seabream (Sparus aurata L.) were studied.

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Article Synopsis
  • - The study investigated how different levels of honey bee pollen (HBP) in the diet affect meagre fish juveniles in terms of growth, digestibility, and gut health.
  • - Results showed that higher HBP inclusion (especially at 4%) led to poor growth rates and negative changes in intestinal structure, highlighting potential irritation caused by the pollen.
  • - Biochemical analysis revealed increased stress markers and a drop in protein levels in fish fed HBP, suggesting that the structural properties of bee pollen might hinder nutrient absorption in these carnivorous fish.
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Microbiological, nutritional and bioactive properties of edible powders obtained from (house cricket) and (mealworm) were investigated. Except for the enterobacteria, viable bacteria were at a higher concentration in mealworm flour. The diversity evaluation carried out using MiSeq Illumina that mainly identified and in mealworm powder and members of the family in house cricket powder.

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Article Synopsis
  • Analysis of the GP's seeds and skins revealed they are rich in polyunsaturated fatty acids and polyphenols, which exhibit strong antioxidant properties.
  • The findings suggest that using GP in nutraceutical applications could enhance sustainability while making effective use of this agricultural waste.
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Article Synopsis
  • The study focuses on extracting the carotenoid astaxanthin from shrimp by-products using supercritical fluid extraction (SFE) and evaluating its bioactive properties in both normal and cancerous human cell lines.
  • In fibroblast cells, the extracted astaxanthin significantly increased cell viability in a dose-dependent manner, demonstrating its antioxidant effects, while in hepatoma cells, it reduced cell vitality in a similar dependent manner, indicating anti-proliferative effects.
  • The research reveals that astaxanthin may offer pro-apoptotic and anti-cancer benefits in treating liver cancer cells, suggesting the potential for utilizing marine by-products in nutraceutical and pharmaceutical sectors without harmful solvents.
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This study aimed to produce new value-added products from dolphinfish () as fillets when using cold smoking combined with natural antioxidants, obtained from a plant belonging to halophytes. The fillet treatments were controlled (untreated), immersed in standard brine (STD), treated with NaCI and antioxidant before freezing (Aox-pre) and treated with NaCl and antioxidant after thawing (Aox-post). The quality of dolphinfish fillets was assessed using sensory, biochemical, microbiological, and technological parameters.

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This study aimed at the characterization of the antioxidant power of polyphenol extracts (PE) obtained from the algae (CYS) (Phaeophyta) and from the halophyte (HAL), growing in the solar saltworks of western Sicily (Italy), and at the evaluation of their anti-microfouling properties, in order to correlate these activities to defense strategies in extreme environmental conditions. The antioxidant properties were assessed in the PE based on the total antioxidant activity test and the reducing power test; the anti-microfouling properties of the two PE were evaluated by measuring the growth inhibition of marine fish and shellfish pathogen bacteria as well as marine surface fouling bacteria and microalgae exposed to the fractions. Similar polyphenol content (CYS 5.

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