Recombinant proteins represent one of the greatest achievements of modern pharmaceutical biotechnology, as they are increasingly used across nearly all branches of medicine to treat a wide range of conditions. In response to this demand, various cell engineering approaches have been developed to improve their expression. Some of these approaches involve the use of genetic elements that prevent the silencing of the gene of interest, as well as the generation of resistant cell lines to inhibit or avoid programmed cell death (PCD).
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