Comparison of lateral tail vein and retro-orbital venous sinus as routes of inoculation to study Group B streptococcal systemic infection.

Unlabelled: Murine models are commonly used to understand pathogen and host determinants of systemic infection. While these models have proven beneficial for uncovering bacterial mechanisms required for progression to invasive disease, it can be challenging to draw comparisons across studies as several different routes of infection are standardly used for these experiments. In this study, one of the leading bacterial meningeal pathogens, , or Group B (GBS), was used to compare experimental outcomes of two commonly used routes of hematogenous infection, lateral tail vein injection and retro-orbital venous sinus injection.

Enhanced Vulnerability of Diabetic Mice to Hypervirulent ST-17 Infection.

(Group B , GBS) is the leading cause of neonatal sepsis and meningitis but has been recently isolated from non-pregnant adults with underlying medical conditions like diabetes. Despite diabetes being a key risk factor for invasive disease, the pathological consequences during GBS infection remain poorly characterized. Here, we demonstrate the pathogenicity of the GBS90356-ST17 and COH1-ST17 strains in streptozotocin-induced diabetic mice.

Wavelength encoded analytical imaging and fiber optic sensing with pH sensitive CdTe quantum dots.

CdTe quantum dots (QDs), capped with mercaptopropionic acid (MPA), were synthesized and the variation of their fluorescence properties (steady state and lifetime) with pH was assessed in solution and when immobilized in a sol-gel host. Three different sizes of CdTe QDs with excited state lifetimes ranging from 42 to 48 ns and with emission maximum at 540 nm (QD(540)), 580 nm (QD(580)) and 625 nm (QD(625)) were selected. The solution pH affects the maximum emission wavelength (shifts to higher wavelengths of 23, 24 and 27 nm for QD(540), QD(580) and QD(625), respectively), the excited state lifetime and the fluorescence intensity in a reversible way.

Multiway chemometric decomposition of EEM of fluorescence of CdTe quantum dots obtained as function of pH.

The effect of the pH (from 3 to 10) on the excitation emission matrices (EEMs) of fluorescence of CdTe quantum dots (QDs), capped with mercaptopropionic acid (MPA), were analyzed by multiway decomposition methods of parallel factor analysis (PARAFAC), a variant of the parallel factor analysis method (PARAFAC2) and multivariate curve resolution alternating least squares (MCR-ALS). Three different sized CdTe QDs with emission maximum at 555 nm (QDa), 594 nm (QDb) and 628 nm (QDc) were selected for analysis. The three-way data structures composed of sets of EEMs obtained as function of the pH (EEMs, pH) do not have a trilinear structure.

PARAFAC analysis of the quenching of EEM of fluorescence of glutathione capped CdTe quantum dots by Pb(II).

Glutathione capped CdTe quantum dots (QD) were synthesised using a simple experimental procedure and two samples were subjected of study (QD550 and QD600). The maximum of the excitation and emission spectra and the emission full width of half maximum of these two QD were: QD550, 307, 550 and 37 nm; QD600, 307, 600 and 39 nm. The steady state fluorescence properties of the two QD undergo variation when the pH of the aqueous solution is varied and are characterised by different apparent pKa: QD550, 5.