Publications by authors named "Colt Capan"
Glucose is essential for T cell proliferation and function, yet its specific metabolic roles remain poorly defined. Here, we identify glycosphingolipid (GSL) biosynthesis as a key pathway fueled by glucose that enables CD8 T cell expansion and cytotoxic function . Using C-based stable isotope tracing, we demonstrate that CD8 effector T cells use glucose to synthesize uridine diphosphate-glucose (UDP-Glc), a precursor for glycogen, glycan, and GSL biosynthesis.
View Article and Find Full Text PDFMetabolite extraction is the critical first-step in metabolomics experiments, where it is generally regarded to inactivate and remove proteins. Here, arising from efforts to improve extraction conditions for polar metabolomics, we discover a proteomic landscape of over 1000 proteins within metabolite extracts. This is a ubiquitous feature across several common extraction and sample types.
View Article and Find Full Text PDFDuring maturation oocytes undergo a recently discovered mitochondrial proteome remodeling event in flies, frogs, and humans. This oocyte mitochondrial remodeling, which includes substantial changes in electron transport chain (ETC) subunit abundance, is regulated by maternal insulin signaling. Why oocytes undergo mitochondrial remodeling is unknown, with some speculating that it might be an evolutionarily conserved mechanism to protect oocytes from genotoxic damage by reactive oxygen species (ROS).
View Article and Find Full Text PDFCytokine and Reward Circuitry Relationships in Treatment-Resistant Depression.
Biol Psychiatry Glob Open Sci
January 2022
Background: Depressive disorders are linked to dysfunction in reward-related behaviors and corticostriatal reward circuitry. Low-grade dysregulation of the immune system, e.g.
View Article and Find Full Text PDFArticle Synopsis
- A study indicates that excess iron accumulation in the substantia nigra, a brain region affected in Parkinson's disease (PD), is linked to the loss of dopamine-producing neurons.
- Researchers found that PD patients had significantly lower levels of plasma serotonin and higher iron levels in the substantia nigra compared to controls.
- There is a notable correlation in PD patients between lower serotonin levels and higher nigral iron, suggesting a potential relationship that could aid understanding of early PD progression.
Background: The objective of this study was to determine whether neurotoxic kynurenine metabolites, induced by inflammation, in plasma and cerebrospinal fluid (CSF) are associated with symptom severity and nigral pathology in Parkinson's disease (PD).
Methods: Clinical and MRI data were obtained from 97 PD and 89 controls. We used ultra-performance liquid chromatography to quantify kynurenine metabolites and high-sensitivity multiplex assays to quantify inflammation in plasma and CSF.