is dispensable for zygotic genome activation but essential for morula development.

Early embryogenesis is driven by transcription factors (TFs) that first activate the zygotic genome and then specify the lineages constituting the blastocyst. Although the TFs specifying the blastocyst's lineages are well characterized, those playing earlier roles remain poorly defined. Using mouse models of the TF , we show that embryos arrest at the early morula stage and exhibit altered lineage specification, frequent mitotic failure, and substantial chromosome segregation defects.

A Dual-Mode Near-Infrared Optical and Photoacoustic Imaging Agent Based on a Low Energy Absorbing Ytterbium Complex.

Near-infrared (NIR) luminescence and photoacoustic (PA) imaging have attracted increasing attention for the real-time monitoring of biological samples due to high sensitivity, resolution, and pronounced signal detection depth, respectively. For improved contrast, both techniques require imaging agents possessing high absorption in the red-NIR range. Herein, we took advantage of a ternary complex formed with the anionic ytterbium(III) (2-thenoyltrifluoroacetonate) ([Yb(tta)]) and the cationic NIR-absorbing chromophore, 1,1'-diethyl-2,2'-dicarbocyanine (Cy), to evaluate its potential to act as a dual-mode NIR luminescence and PA imaging agent.

Biocatalytic Synthesis of Coumarin -Glycosides: Towards Non-Cytotoxic Probes for Biomedical Imaging and Sensing.

This study unveils an innovative method for synthesizing coumarin -glycosides, employing original biocatalysts able to graft diverse carbohydrate structures onto 7-mercapto-4-methyl-coumarin in one-pot reactions. The fluorescence properties of the generated thio-derivatives were assessed, providing valuable insights into their potential applications in biological imaging or sensing. In addition, the synthesized compounds exhibited no cytotoxicity across various human cell lines.

Typical Bone Scintigraphy Presentation of Erdheim-Chester Disease in a Patient Diagnosed With IgG4-Related Disease.

A 50-year-old woman presented a dry syndrome, joint pain, inflammatory syndrome, polyclonal hypergammaglobulinemia, and tubulointerstitial nephritis. Imaging studies (including FDG PET/CT) revealed infrarenal retroperitoneal fibrosis with periaortitis and hypermetabolic osteosclerotic lesions. Bone scintigraphy demonstrated intense uptake in the femoral, tibial, and radial regions, suggestive of non-Langerhans histiocytosis, specifically Erdheim-Chester disease.

Multi-Technique Characterization of 3.45 Ga Microfossils on Earth: A Key Approach to Detect Possible Traces of Life in Returned Samples from Mars.

The NASA Mars 2020 Perseverance rover is actively exploring Jezero crater to conduct analyses on igneous and sedimentary rock targets from outcrops located on the crater floor (Máaz and Séítah formations) and from the delta deposits, respectively. The rock samples collected during this mission will be recovered during the Mars Sample Return mission, which plans to bring samples back to Earth in the 2030s to conduct in-depth studies using sophisticated laboratory instrumentation. Some of these samples may contain traces of ancient martian life that may be particularly difficult to detect and characterize because of their morphological simplicity and subtle biogeochemical expressions.

Regulus infers signed regulatory relations from few samples' information using discretization and likelihood constraints.

Motivation: Transcriptional regulation is performed by transcription factors (TF) binding to DNA in context-dependent regulatory regions and determines the activation or inhibition of gene expression. Current methods of transcriptional regulatory circuits inference, based on one or all of TF, regions and genes activity measurements require a large number of samples for ranking the candidate TF-gene regulation relations and rarely predict whether they are activations or inhibitions. We hypothesize that transcriptional regulatory circuits can be inferred from fewer samples by (1) fully integrating information on TF binding, gene expression and regulatory regions accessibility, (2) reducing data complexity and (3) using biology-based likelihood constraints to determine the global consistency between a candidate TF-gene relation and patterns of genes expressions and region activations, as well as qualify regulations as activations or inhibitions.

Multigene Panel Sequencing Identifies a Novel Germline Mutation Profile in Male Breast Cancer Patients.

Even though male breast cancer (MBC) risk encompasses both genetic and environmental aetiologies, the primary risk factor is a germline pathogenic variant (PV) or likely pathogenic variant (LPV) in and/or genes. To identify new potential MBC-specific predisposition genes, we sequenced a panel of 585 carcinogenesis genes in an MBC cohort without PV/LPV. We identified 14 genes carrying rare PVs/LPVs in the MBC population versus noncancer non-Finnish European men, predominantly coding for DNA repair and maintenance of genomic stability proteins.

[The role of physicians in patient and family adherence to genetic testing].

Introduction: More and more French cancer patients are offered by their physicians having their genetic characteristics analyzed (diagnosis, adaptation of treatment plans, etc.). In oncology, considering the development of personalized medicine, these analyses are commonplace.

Near-Infrared Emitting Poly(amidoamine) Dendrimers with an Anthraquinone Core toward Versatile Non-Invasive Biological Imaging.

Today, there is a very strong demand for versatile near-infrared (NIR) imaging agents suitable for non-invasive optical imaging in living organisms ( imaging). Here, we created a family of NIR-emitting macromolecules that take advantage of the unique structure of dendrimers. In contrast to existing fluorescent dendrimers bearing fluorophores at their periphery or in their cavities, a NIR fluorescent structure is incorporated into the core of the dendrimer.

Fluorodeoxyglucose positron emission tomography-computed tomography findings in a first series of 10 patients with polyarteritis nodosa.

Objective: 18F-fluorodeoxyglucose PET/CT (FDG-PET/CT) is widely used in patients with large vessel vasculitis. The benefits of FDG-PET/CT in PAN has only ever been assessed in three case reports. Our aim was to describe FDG-PET/CT findings in 10 patients with newly diagnosed PAN.

A near-infrared emitting MOF: controlled encapsulation of a fluorescein sensitizer at the time of crystal growth.

We report here a near-infrared (NIR) emitting lanthanide-based metal-organic framework (MOF) in which Yb are sensitized by fluorescein (FL) as a low energy absorbing chromophore (FL@CD-MOF-161). The unique design of CD-MOF-161 allows for the entrapment of FL molecules in its pores during the synthesis and crystal growth, ensuring the efficient loading and spreading of chromophores within the crystal volume.

Tumour angiogenesis normalized by myo-inositol trispyrophosphate alleviates hypoxia in the microenvironment and promotes antitumor immune response.

Pathologic angiogenesis directly responds to tumour hypoxia and controls the molecular/cellular composition of the tumour microenvironment, increasing both immune tolerance and stromal cooperation with tumour growth. Myo-inositol-trispyrophosphate (ITPP) provides a means to achieve stable normalization of angiogenesis. ITPP increases intratumour oxygen tension (pO ) and stabilizes vessel normalization through activation of endothelial Phosphatase-and-Tensin-homologue (PTEN).

Auditory processing disorder in children: the value of a multidisciplinary assessment.

Purpose: Auditory processing disorder (APD) may affect 0.2-5% of the paediatric population. The diagnosis of APD remains difficult because of polymorphic symptoms possibly entangled with other difficulties.

COVID-19 pandemic and pediatric dentistry: Fear, eating habits and parent's oral health perceptions.

Background: Severe measures have been implemented around the world to reduce COVID-19 spread with a significant impact on family dynamics.

Aim: To assess the impact of the pandemic on fear, dietary choices and oral health perceptions of parents.

Design: questionnaire containing 19 questions was remotely applied to 1003 parents of children aged 0-12 years.

[Intravascular large B cell lymphoma pathological findings led by positron emission tomography findings: About one case].

Intravascular large B cell lymphoma is a rare non-Hodgkin large B cell lymphoma disease, with heterogeneous clinical manifestation and difficult pathological diagnosis. Positron emission tomography may be helpfull in this context and has already been reported. A 45-year-old woman was admitted for persistent high fever, inflammatory syndrome and unexplained haemophagocytic syndrome.

Ship-in-a-Bottle Preparation of Long Wavelength Molecular Antennae in Lanthanide Metal-Organic Frameworks for Biological Imaging.

While metal-organic frameworks (MOFs) have been identified as promising materials for sensitizing near-infrared emitting lanthanide ions (Ln) for biological imaging, long-wavelength excitation of such materials requires large, highly delocalized organic linkers or guest-chromophores. Incorporation of such species generally coincides with fewer Ln emitters per unit volume. Herein, the excitation bands of ytterbium-based MOFs are extended to 800 nm via the postsynthetic coupling of acetylene units to form a high density of conjugated π-systems throughout MOF pores.

Iodinated Metallacrowns: Toward Combined Bimodal Near-Infrared and X-Ray Contrast Imaging Agents.

Multimodal probes capable of combining imaging modalities within a single molecule are in high demand today as they can provide information at both molecular and anatomical levels. Herein, a study was conducted on a series of gallium(III)/lanthanide(III) bis(12-MC-4) metallacrowns (MCs) with the general composition {Ln[12-MC -4]} (iph) (Ln-I , x=0, 4, 8, 12), where shi and iph are salicylhydroximate and isophthalate ligands, respectively, or their iodinated derivatives. For Yb-I , the attenuation in X-ray computed tomography (XCT) imaging and near-infrared (NIR) luminescence properties can be finely tuned by controlled structural modifications based on iodo groups.

Remediation of Allophonic Perception and Visual Attention Span in Developmental Dyslexia: A Joint Assay.

Categorical perception of phonemes and visual attention span are cognitive processes that contribute independently to poor reading skills in developmental dyslexia. We here explored whether training programs specifically targeting one or the other process do improve reading performance in dyslexic children. The dyslexic participants were trained using either the RapDys© program designed to improve phonemic perception or the MAEVA© program targeting visual attention span.

One Approach for Two: Toward the Creation of Near-Infrared Imaging Agents and Rapid Screening of Lanthanide(III) Ion Sensitizers Using Polystyrene Nanobeads.

The unique luminescence properties of lanthanide(III) ions (Ln) in the near-infrared (NIR) range are attracting major attention in view of their exciting applications in the fields of technology, telecommunications, biology, and medicine. One of the main strategies to design luminescent Ln-based compounds relies on their sensitization through appropriate chromophoric ligands. The choice of the chromophores depends on the nature of Ln and, as of today, still partially requires experimental trials, in particular, for the creation of luminescent compounds incorporating NIR-emitting Ln.

Arterial Phase 18F-Fluorocholine PET/CT in Hepatocellular Carcinoma.

A 57-year-old man was referred to our institution for F-fluorocholine PET/CT to characterize a pulmonary nodule in a context of hepatocellular carcinoma. F-FDG PET/CT did not show any uptake of the pulmonary nodule. F-fluorocholine PET/CT showed high uptake of the pulmonary nodule, confirming its metastatic origin.

Heteroleptic Ter-Bidentate Cr(III) Complexes as Tunable Optical Sensitizers.

To exploit Cr(III) coordination complexes as sensitizers in supramolecular energy-converting devices, the latter optical relays should display long-lived excited states, broad emission bands, and tunable spatial and electronic connections to activator units. An ad-hoc versatile strategy has been therefore developed for the preparation of a family of luminescent pseudo-octahedral [CrN] chromophores made up of ter-bidentate heteroleptic [Cr(phen)(N-N'')] complexes, where phen is 1,10-phenanthroline, and N-N' stands for α,α'-diimine ligands possessing peripheral substituents compatible with both electronic tuning and structure extensions. As long as the ligand field in these [CrN] chromophores remains sufficiently strong to avoid back-intersystem crossing, photophysical studies indicate that the lifetime of the near-infrared emissive Cr(E) excited state is poorly sensitive to ligand-based electronic effects.