Publications by authors named "Collenot A"

A mutation which affects the eggs was detected in a Pleurodeles waltl laboratory strain. About 4 to 5 hours after fertilization, i.e.

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In Pleurodeles waltl, progeny resulting from a cross between 2 individuals of the Z/W sexual genotype include 25% of W/W individuals, while those issued from crossing a Z/W neomale with a W/W thelygenous female include 50% of W/W individuals. W/W individuals can be identified through the peptidase-1 zymogram since, in P. waltl, this enzyme is controlled by codominant alleles which are linked to the sex chromosomes.

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In Pleurodeles waltlii, peptidase-1 is a dimeric enzyme which has been shown to be controlled by two codominant alleles, Pep-1A and Pep-1B (Ferrier et al., 1980, 1983), linked respectively to the Z and W sex chromosomes. The enzymatic patterns obtained after starch gel electrophoresis were used routinely to identify the sexual genotypes of animals reared in our laboratory.

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With the potassium ferricyanide method, the localization of the sites of 3 beta-hydroxysteroid dehydrogenase activity has been investigated in the glandular tissue cells, previously fixed, of the Pleurodeles testis. An abundant copper ferrocyanide precipitate, the final reaction product, is observed in the vicinity of the external faces of the membranes of the smooth endoplasmic reticulum, or in contact with them. A very weak reaction occurs in mitochondrial cristae.

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