Accounting for variability when resurrecting dormant propagules substantiates their use in eco-evolutionary studies.

There has been a steady rise in the use of dormant propagules to study biotic responses to environmental change over time. This is particularly important for organisms that strongly mediate ecosystem processes, as changes in their traits over time can provide a unique snapshot into the structure and function of ecosystems from decades to millennia in the past. Understanding sources of bias and variation is a challenge in the field of resurrection ecology, including those that arise because often-used measurements like seed germination success are imperfect indicators of propagule viability.

A century-long record of plant evolution reconstructed from a coastal marsh seed bank.

Evidence is mounting that climate-driven shifts in environmental conditions can elicit organismal evolution, yet there are sparingly few long-term records that document the tempo and progression of responses, particularly for plants capable of transforming ecosystems. In this study, we "resurrected" cohorts of a foundational coastal marsh sedge () from a time-stratified seed bank to reconstruct a century-long record of heritable variation in response to salinity exposure. Common-garden experiments revealed that exhibits heritable variation in phenotypic traits and biomass-based measures of salinity tolerance.

Applied use of alternate stable state modeling in restoration ecology.

The concept of alternate stable states is important in ecological theory and models, but the application and implementation of these models have the potential to make significant future advances in the field of patterned landscapes. The bistable ridge and slough landscape is a central feature of Everglades restoration and provides an important opportunity to test stable state theory with multistate transition models. We used these models to estimate environmental parameters associated with state changes (water depths, edaphic factors, etc.

A century of genetic variation inferred from a persistent soil-stored seed bank.

Stratigraphic accretion of dormant propagules in soil can result in natural archives useful for studying ecological and evolutionary responses to environmental change. Few attempts have been made, however, to use soil-stored seed banks as natural archives, in part because of concerns over nonrandom attrition and mixed stratification. Here, we examine the persistent seed bank of , a foundational brackish marsh sedge, to determine whether it can serve as a resource for reconstructing historical records of demographic and population genetic variation.