Low-cost, open-source cell culture chamber for regulating physiologic oxygen levels.

The physiological oxygen levels for several mammalian cell types are considered to be hypoxic (low oxygen tension), but the vast majority of mammalian cell culture is conducted at atmospheric oxygen levels of around 21%. In order to understand the impact of low oxygen environments on cells, oxygen levels need to be regulated during culture. Two common methods for simulating a hypoxic environment are through the regulation of gas composition or chemical induction.