Publications by authors named "Colin James Bent"
Biotechnol Prog
March 2021
Article Synopsis
- Ultrafiltration/diafiltration (UF/DF) is a key step in protein drug manufacturing aimed at concentrating protein solutions, but often results in unwanted changes in pH and composition that can affect drug stability and performance.
- The observed shifts can be explained by Donnan and volume exclusion effects, and a new mechanistic model has been developed to predict and counter these changes based on protein properties and membrane conditions.
- This model has shown strong predictive capabilities through various tests with monoclonal antibodies, indicating that pH and excipient concentration shifts are notably affected by protein concentration and ionic strength, making it a valuable tool for improving UF/DF processes.
