Scanning electron microscopic study of leukemic human B lymphocytes.

Peripheral blood lymphocytes from patients with chronic lymphocytic leukemia (CLL), lymphoplasmacytoid lymphoma, centrocytic lymphoma and hairy cell leukemia were studied by scanning electron microscopy (SEM). In general, SEM revealed rather homogenous cell populations. Most lymphocytes displayed a moderately villous surface architecture, although smooth surfaces predominated in 3 cases with CLL and in 1 case with lymphoplasmacytoid lymphoma.

[T-cells in thyroid disease (author's transl)].

Thymus-derived peripheral blood lymphocytes were studied in untreated (n = 18), methimazole-treated (n = 28) thyrotoxicosis, after radioiodine (n = 14), in Hashimoto thyroiditis (n = 7) and in euthyroid goiter (n = 7). The results were compared with normal persons (n = 40) without thyroid disease. There was no significant difference in the total and relative counts of T-cells either between the different groups nor compared with the controls.

[Transitory monoclonal gammaopathy after smallpox vaccination].

The case is described of a 51-year-old female who developed monoclonal gammopathy of IgMk-type following smallpox vaccination. The Mcomponent was detectable, albeit in decreasing concentrations, over a period of 10 months and then disappeared spontaneously. The findings are discussed in relation to "essential" paraproteinemia and the literature on transient monoclonal gammopathy is reviewed.

Distribution of erythrocyte receptors at the surface membrane of human T-lymphocytes.

During incubation at 4 degrees C, the binding sites for neuraminidase-treated human erythrocytes (nHRBC) and sheep red blood cells (SRBC) were diffusely distributed on the surface membrane of most rosette-forming normal human blood lymphocytes. Increasing the temperature to 37 degrees C resulted in a rapid rosette dissociation which was accompanied by a relative increase in cap formation and a corresponding decrease of ring-like rosettes. The conversion into caps was inhibited by reincubation of the cells at 4 degrees C.

Cell numbers in human lymphocyte cultures stimulated with pokeweed mitogen.

As determined by electronic cell counting, the cell numbers in pokeweed mitogen (PWM) stimulated cultures of normal lymphocytes decreased by about 13% during the first day and then remained almost constant up to day 8. In contrast, a progressive decrease of the cell count was observed in cultures of chronic lymphocytic leukaemia (CLL) lymphocytes reaching about 40% of the initial number on day 8. In PWM cultures of normal lymphocytes the transformed cells increased to about 20% of the cells present on day 4, whereas in cultures of CLL lymphocytes these cells reached only 11% on day 7.

Studies on human T lymphocyte rosette formation with human and sheep erythrocytes.

A large proportion of normal human blood lymphocytes forms rosettes with neuraminidase-treated autologous and allogeneic human erythrocytes (nHRBC) or sheep red blood cells (SRBC). In contrast, the percentage of rosette-forming cells is extremely reduced in the peripheral blood of patients with chronic lymphocytic leukemia. The formation of mixed rosettes after addition of nHRBC and SRBC suggests that most normal rosette-forming lymphocytes have receptor sites for both types of erythrocytes.

[Early CNS manifestations in lupus erythematosus (author's transl)].

CNS involvement is a rare manifestation of collagen disease. But in three patients with lupus erythematosus (L.E.

Human T-lymphocyte rosette formation: inhibition by cytochalasin B.

The effects of cytochalasin B, colchicine and vinblastine on the rosette formation of human T lymphocytes with neuraminidase-treated human erythrocytes (nHRBC) and with sheep red blood cells (SRBC) have been studied. Pretreatment of the lymphocytes with cytochalasin B which affects microfilament action reversibly inhibits both nHRBC and SRBC rosette formation. Colchicine and vinblastine known to interact with microtubules causes no major reduction in rosette-forming cells.

Peripheral blood T and B lymphocytes in men in different age groups.

Peripheral blood T and B lymphocytes were determined in normal humans at different ages. Spontaneous rosette formation with sheep red blood cells (SRBC) was used as a marker for T cells. B cells were detected by immunofluorescent staining of membrane-bound immunoglobulins.

[Lymphocyte in chronic lymphatic leukemia].

In patients with chronic lymphatic leukemia (CLL) the majority of peripheral blood lymphocytes was characterized as B cells by surface membrane markers. Ultrastructural studies revealed a reduction of the cytoplasmic area. Furthermore, the number of lysosomes was diminished corresponding to a decreased activity of lysosomal hydrolases.

[T and B lymphocytes in chronic lymphocytic leukemia and Hodgkin's disease. Electron microscopic and immunologic studies].

The number of lymphocytes forming spontaneous rosettes with sheep erythrocytes, a property of thymus-dependent (T) cells, and the number of lymphocytes bearing surface immunoglobulins, a characteristic feature of bone marrow-dependent (B) cells, were determined in the peripheral blood of normals and of patients with chronic lymphocytic leukemia (CLL) and Hodgkin's disease. As compared with normal individuals CLL patients had an increased percentage of lymphocytes with membrane-bound immunoglobulins, whereas the proportion of rosette-forming lymphocytes was reduced. In Hodgkin's disease either normal, diminished, or increased B cell values were obtained; the percentage of T cells was decreased or within the lower range of normals.