Towards epigenetic regulation of triple-negative breast cancer via ligand-mediated nanoparticles.

Develop and analyze triple-negative breast cancer targeted nanoparticles loaded with the demethylating agent decitabine. The polymers were synthesized by ring-opening polymerization of D,L-lactide and formulated into nanoparticles via emulsion-evaporation method. The nanoparticles were characterized by physicochemical analysis as well as using breast cancer cell lineages.

Oxygen therapy alternatives in COVID-19: From classical to nanomedicine.

Around 10-15% of COVID-19 patients affected by the Delta and the Omicron variants exhibit acute respiratory insufficiency and require intensive care unit admission to receive advanced respiratory support. However, the current ventilation methods display several limitations, including lung injury, dysphagia, respiratory muscle atrophy, and hemorrhage. Furthermore, most of the ventilatory techniques currently offered require highly trained professionals and oxygen cylinders, which may attain short supply owing to the high demand and misuse.

Supercritical Fluids: A Promising Technique for Biomass Pretreatment and Fractionation.

Lignocellulosic biomasses are primarily composed of cellulose, hemicelluloses and lignin and these biopolymers are bonded together in a heterogeneous matrix that is highly recalcitrant to chemical or biological conversion processes. Thus, an efficient pretreatment technique must be selected and applied to this type of biomass in order to facilitate its utilization in biorefineries. Classical pretreatment methods tend to operate under severe conditions, leading to sugar losses by dehydration and to the release of inhibitory compounds such as furfural (2-furaldehyde), 5-hydroxy-2-methylfurfural (5-HMF), and organic acids.

Influence of mechanical pretreatment to isolate cellulose nanocrystals by sulfuric acid hydrolysis.

The mechanical pretreatments intensities on characteristics of cellulose nanocrystals (CNC) prior to acid hydrolysis was evaluated. The cellulose was submitted to mechanical pretreatment as: magnetic stirring (CNC), blending (CNC) or grinding by 20 (CNC) and 40 (CNC) passages in a super mass colloid mill. Then, all samples were submitted to HSO hydrolysis and the CNC were evaluated by total mass yield (TMY%), rheological behavior, size distribution for width/length (WD), crystallinity index (CI%), OSO substitution degree (SD) and zeta potential (ζ).

Bacterial cellulose in biomedical applications: A review.

Bacterial cellulose (BC) derived materials represents major advances to the current regenerative and diagnostic medicine. BC is a highly pure, biocompatible and versatile material that can be utilized in several applications - individually or in the combination with different components (e.g.

Piezoelectric immunochip coated with thin films of bacterial cellulose nanocrystals for dengue detection.

Low-cost piezoelectric devices, such as simple frequency monitoring quartz crystal microbalance (QCM) devices, have good clinical utility as fast diagnostic tools for the detection of several diseases. However, unspecific antigen recognition, poor molecular probe adsorption and the need for sample dilution are still common drawbacks that hinder their use in routine diagnosis. In this work, piezoelectric sensors were previously coated with thin films of bacterial cellulose nanocrystals (CN) to provide a more sensitive and adapted interface for the attachment of monoclonal immunoglobulin G (IgGNS1) and to favor specific detection of non-structural protein 1 (NS1) of dengue fever.

Chitosan-coated microvesicles: Effect of polysaccharide-phospholipid affinity on decafluorobutane dissolution.

The stability of perfluorinated microvesicles is mainly determined by the presence of interfacial materials and their ability to hinder the gas component diffusibility into the bloodstream. The goal of this study is to increase the persistence of the gaseous-core by introducing chitosan-coated 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) microvesicles, reducing gas diffusion from microvesicles, and increasing for a long time ultrasonic signals. Our hypothesis was based on the irreversible adhesion of chitosan towards DSPC head groups observed in thin-films models.

Hydrophilicity improvement of mercerized bacterial cellulose films by polyethylene glycol graft.

In this work, polyethylene glycol (PEG), of tree distinct molar masses (200, 300 and 400 g mol(-1)), was grafted onto mercerized bacterial nanocellulose (BNCm) and applied to produce nanofilms (BNCm-PEG). The products BNCm-PEG were characterized by NMR and thermal analysis. Solid-state NMR and X-ray diffraction analyses exhibited no significant differences in index of BNCm-PEG derivatives compared to BNCm, indicating that grafting reaction did not modify the BNCm crystalline structure.