Transcriptomic identification of genes associated with thrombosis and coagulation in lipopolysaccharide-exposed bovine monocyte-derived macrophages.

Objective: The objective of this study was to investigate the differential expression of genes associated with coagulation in bovine monocyte-derived macrophages (MoMΦ) exposed to lipopolysaccharide (LPS) in vitro. We hypothesized that MoMΦ stimulated with LPS would have upregulation of procoagulant genes and downregulation of genes protecting against coagulation.

Methods: MoMΦ were isolated from Holstein steers and exposed to Escherichia coli-derived LPS or a control for 3 hours.

Effect of arginine, glycine + serine concentrations, and guanidinoacetic acid supplementation in vegetable-based diets for chickens.

The study investigated guanidinoacetic acid (GAA) supplementation with varying dietary digestible arginine (Arg) and glycine+serine (Gly+Ser) concentrations in the starter phase, exploring respective carry-over effects on growth performance, blood chemistry, incidence of pectoral myopathies and proximate composition in broilers. A total of 2,800 one-day-old male broiler chicks were distributed in a central composite design with 2 factors and double experimental mesh, represented by supplementation or omission of 0.6 g per kg of GAA, with a central point represented by 107% of Arg and 147% of Gly+Ser, 4 factorial points (combinations of Arg/Gly+Ser concentrations: 96.

Use of RNA-seq to identify genes encoding cytokines and chemokines activated following uptake and processing a candidate peptide vaccine developed against subsp. .

Analysis of the primary and recall responses to a membrane molecule (MMP), encoded by MAP2121c demonstrated that tri-directional signaling between the antigen-presenting cell (APC), CD4 and CD8 is essential for eliciting a CD8 cytotoxic T cell (CTL) response against Mycobacterium avium subsp. paratuberculosis. As reported here, RNA-sequencing was used to initiate the characterization of the signaling pathways involved in eliciting the development of CD8 CTL, starting with the characterization of the activation status of genes in monocyte-derived macrophages (MoMΦ) following uptake and processing MMP for the presentation of antigenic epitopes to CD4 and CD8 T cells.

Cytologic features of canine melanotroph and corticotroph pituitary adenomas.

Background: The introduction of intraoperative cytology revolutionized neurosurgical procedures in human medicine, providing real-time diagnostic guidance to surgeons and contributing to improved patient outcomes. In the realm of veterinary medicine, the understanding of pituitary tumors in dogs and cats remains limited due to challenges in obtaining antemortem samples of central nervous system lesions.

Objectives: The aim of this study was to describe the cytologic features of pituitary adenomas in 12 dogs that underwent hypophysectomy.

Applying different morphometric intestinal mucosa methods and the correlation with broilers performance under Eimeria challenge.

The intestinal wall has on its surface, protrusions called villi that are responsible for the absorption of nutrients. Commonly, these structures have their dimensions measured to related more area surface with better absorption. However, the measurement of these villi neglects the inflammation and the presence of immature cells that increase the surface area but affect negatively the absorption and compromise the animal performance.

Influence of dietary corn distillers' dried grains with solubles on nutrient digestibility, growth performance, and carcass traits in rabbits.

Two experiments were performed to evaluate the digestibility and growth performance of New Zealand White rabbits fed corn distillers' dried grains with solubles (DDGS). For the digestibility trial, 20 rabbits were housed in metabolic cages. The animals were distributed in two treatments, T1 (control diet without inclusion of DDGS) and T2 (control diet + 300 g kg DDGS).

Salmon poisoning disease in dogs: clinical presentation, diagnosis and treatment.

Salmon poisoning disease (SPD) is caused by a rickettsial organism, that is carried by the trematode which encysts in freshwater fish, most commonly salmonids. We reported two dogs from the United States West Coast that had similar clinical signs, hematologic and biochemistry findings. They were both diagnosed with salmon poisoning disease.

Broiler behavior differs from males to females when under different light wavelengths.

It is well established that different light wavelengths affect broiler behavior. The present study aims to evaluate the effect of four light wavelengths on broiler behavior from 1 to 42 days of age. Birds were housed at a stocking density of 13 birds/m, in 32 boxes of 1.

Effect of environmental enrichment for piglets in the nursery phase.

Objective: The effect of environmental enrichment on the behaviour of piglets in the nursery phase was evaluated.

Methods: A total of 450 hybrid pigs (21 day old), including both females and uncastrated males, weighing approximately 6 kg, were distributed in a completely randomised design with 3 treatments and 3 replicates of 50 animals each. The treatments were: i) pen without environmental enrichment (control), ii) treatment consisting of continuous environmental enrichment (CEE) with rubber balls throughout the experimental period, and iii) treatment consisting of environmental enrichment with washed balls (EEWB) during the whole experimental period which were removed daily for washing.

Behavioral responses of turkeys subjected to different climatic conditions.

I was evaluated the effect of seven different combinations of temperature, air velocity, and relative air humidity on the frequency and duration of eating, drinking, resting, cannibalism, dust bathing, scratching, ground pecking, shivering, and stretching behaviors of turkeys at three different ages. The combinations tested of temperature, relative air humidity, and air velocity were, respectively: 1 (22 °C, 50%, 1 m/s); 2 (26.2 °C, 73.

Evaluation of different heating systems for new-born swine.

The use of heating systems for new-born piglets can prevent major losses in the first 5 weeks of life. The present study had the objective of evaluating three heating systems in creeps for new-born piglets, being: floor-heated floor with circulating water (HF), incandescent lamp type SPOT 40 W (L) and incandescent lamp type SPOT 40 W plus black metal plate (LP). The variables evaluated were piglet performance (total weight gain and mortality), bioclimatic data (temperature and relative air humidity), animal behaviour and economic revenue.

A critical role of interleukin-10 in the response of bovine macrophages to infection by Mycobacterium avium subsp paratuberculosis.

Objectives: To evaluate the role of interleukin (IL)-10 in the inability of monocyte-derived bovine macrophages to kill Mycobacterium avium subsp paratuberculosis organisms in vitro.

Sample Population: Monocytes were obtained from healthy adult Holstein dairy cows that had negative results when tested for infection with M avium subsp paratuberculosis.

Procedure: Monocyte-derived macrophages were incubated with M avium subsp paratuberculosis for 2, 6, 24, 72, or 96 hours with or without addition of saturating concentrations of a goat anti-human IL-10 that has been documented to neutralize bovine IL-10 activity.