Sequency encoding single pixel spectroscopy based on Hadamard transform.

Single pixel spectroscopy based on Hadamard transform (SPS-HT) has been applied widely because of its capability of wavelength multiplexing and associated advantage in signal-to-noise ratio. In this paper, we propose a sequency encoding single pixel spectroscopy (SESPS) based on two-dimensional (2D) masks for concurrent coding of all Hadamard coefficients instead of one-dimensional (1D) Hadamard masks (only coding one coefficient at a time) widely used in the traditional SPS-HT. Moreover, each Hadamard coefficient is coded along the time dimension with a different sequency value such that the alternating current (AC) measurements of the time-domain signal can be used to reconstruct all Hadamard coefficients simultaneously, which reduces the influence of noise and dramatically speeds up data acquisition.

Cell Membrane-Coated Electrospun Fibers Enhance Keratinocyte Growth through Cell-Type Specific Interactions.

Although cell membrane-coated fiber scaffolds can be useful for regenerative medicine by presenting both cell surface antigens and topographical cues, it remains unknown whether changes in cellular behavior on cell membrane-coated scaffolds are due to specific cell-cell interactions. In this work, the effects of scaffold fiber diameters and surface charges on the cell membrane coating efficiency were explored. Furthermore, fibroblast membrane-coated scaffolds improved the growth of human keratinocytes as compared to red blood cell membrane-coated and plain scaffolds.

Towards ultrasensitive malaria diagnosis using surface enhanced Raman spectroscopy.

We report two methods of surface enhanced Raman spectroscopy (SERS) for hemozoin detection in malaria infected human blood. In the first method, silver nanoparticles were synthesized separately and then mixed with lysed blood; while in the second method, silver nanoparticles were synthesized directly inside the parasites of Plasmodium falciparum. It was observed that the first method yields a smaller variation in SERS measurements and stronger correlation between the estimated contribution of hemozoin and the parasitemia level, which is preferred for the quantification of the parasitemia level.

Hollow agarose microneedle with silver coating for intradermal surface-enhanced Raman measurements: a skin-mimicking phantom study.

Human intradermal components contain important clinical information beneficial to the field of immunology and disease diagnosis. Although microneedles have shown great potential to act as probes to break the human skin barrier for the minimally invasive measurement of intradermal components, metal microneedles that include stainless steel could cause the following problems: (1) sharp waste production, and (2) contamination due to reuse of microneedles especially in developing regions. In this study, we fabricate agarose microneedles coated with a layer of silver (Ag) and demonstrate their use as a probe for the realization of intradermal surface enhanced Raman scattering measurements in a set of skin-mimicking phantoms.

Recovery of Raman spectra with low signal-to-noise ratio using Wiener estimation.

Raman spectroscopy is a powerful non-destructive technique for qualitatively and quantitatively characterizing materials. However, noise often obscures interesting Raman peaks due to the inherently weak Raman signal, especially in biological samples. In this study, we develop a method based on spectral reconstruction to recover Raman spectra with low signal-to-noise ratio (SNR).

Optimization of Fe3O4@Ag nanoshells in magnetic field-enriched surface-enhanced resonance Raman scattering for malaria diagnosis.

The great potential of magnetic field enriched surface enhanced resonance Raman spectroscopy (SERRS) for early malaria diagnosis has been demonstrated previously. This technique is able to detect β-hematin, which is equivalent to a malaria biomarker (hemozoin) in Raman features, at a concentration of 5 nM. In this study, we present the optimization of nanoparticles used in the magnetic field enriched SERRS by tuning the core size and shell thickness of nanoparticles with an iron oxide core and a silver shell (Fe3O4@Ag).

Towards in vivo intradermal surface enhanced Raman scattering (SERS) measurements: silver coated microneedle based SERS probe.

We propose a microneedle coated with silver (Ag) to detect analytes at low concentrations positioned at a depth of more than 700 μm below the surface of a skin phantom with absorbers and scatterers for mimicking the intradermal surface-enhanced Raman scattering (SERS) measurements. The Ag layer in the Ag-coated microneedle-based probe is found to be the key to the effective detection of analytes buried inside the aforesaid phantom. Glucose concentrations ranging from 5 to 150 mM inside phantoms can be estimated with a root mean square error (RMSE) of 3.

Magnetic field enriched surface enhanced resonance Raman spectroscopy for early malaria diagnosis.

Hemozoin is a by-product of malaria infection in erythrocytes, which has been explored as a biomarker for early malaria diagnosis. We report magnetic field-enriched surface-enhanced resonance Raman spectroscopy (SERRS) of β-hematin crystals, which are the equivalent of hemozoin biocrystals in spectroscopic features, by using magnetic nanoparticles with iron oxide core and silver shell (Fe(3)O(4)@Ag). The external magnetic field enriches β-hematin crystals and enhances the binding between β-hematin crystals and magnetic nanoparticles, which provides further improvement in SERRS signals.

Low-level detection of anti-cancer drug in blood plasma using microwave-treated gold-polystyrene beads as surface-enhanced Raman scattering substrates.

We report on a low-level detection of anti-cancer drug (paclitaxel) in blood plasma using microwave-treated gold (Au) film-polystyrene (PS) beads as surface-enhanced Raman scattering (SERS) substrates. SERS spectra of paclitaxel can be acquired within 10s at a concentration of 1×10(-8) M in blood plasma adsorbed on the modified Au-PS SERS substrates. The concentrations of paclitaxel ranging from 1.

Improving surface-enhanced Raman scattering effect using gold-coated hierarchical polystyrene bead substrates modified with postgrowth microwave treatment.

We report a novel postgrowth microwave heating implementation by selectively modifying hierarchical polystyrene (PS) bead substrates coated with gold (Au) films to effectively improve the surface-enhanced Raman scattering (SERS) effect on the analytes. The SERS signal of probe molecule rhodamine 6G (Rh 6G) on the microwave-treated Au-PS substrates can be improved by 10-fold, while the detection limit of Rh 6G in concentration can be enhanced by two orders of magnitude compared to the as-growth substrates. The high-quality SERS spectrum of saliva can also be acquired using the modified substrates, demonstrating the potential for the realization of the high-performance SERS substrates for biomedical applications.