Use of HO to Cause Oxidative Stress, the Catalase Issue.

Addition of hydrogen peroxide (HO) is a method commonly used to trigger cellular oxidative stress. However, the doses used (often hundreds of micromolar) are disproportionally high with regard to physiological oxygen concentration (low micromolar). In this study using polarographic measurement of oxygen concentration in cellular suspensions we show that HO addition results in O release as expected from catalase reaction.