The purpose of this study was to develop a method for identification of the pylorus, in lactating Holstein cows, based on ultrasound examination and the use of external anatomical landmarks such as the mammary vein . An ultrasound method based on successive identification of 4 internal anatomical landmarks was developed. The procedure was performed 157 times, on clinically healthy lactating Holstein cows, with a portable ultrasound machine equipped with a 3.
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