Identification of an allele of CLA1 associated with variegation in Arabidopsis thaliana.

We have identified a mutant of Arabidopsis thaliana (lvr111) that exhibits a variegated phenotype, reduced isoprenoid pigmentation, and dwarfism in comparison with wild-type plants. Segregation analysis indicated that this phenotype was caused by a single, semi-dominant mutation and PCR-based marker mapping placed the mutation near position 56 on the RI map of chromosome IV. The lvr111 lesion was identified by genomic PCR and sequence analysis as a missense mutation (D306N) in the CLA1 gene (AT4g15560) and complementation analysis confirmed the allelic relationship between lvr111 and CLA1.

Prenylcysteine alpha-carboxyl methyltransferase expression and function in Arabidopsis thaliana.

Farnesylated proteins undergo a series of post-translational modifications, including carboxyl terminal isoprenylation, proteolysis, and methylation. In Arabidopsis thaliana, protein farnesylation has been shown to be necessary for negative regulation of ABA signaling. However, the role of post-isoprenylation protein processing in ABA signal transduction has not been described.